PXD028685

PXD028685 is an original dataset announced via ProteomeXchange.

Title	Comprehensive atlas of the myelin basic protein interaction landscape
Description	Intrinsically disorder myelin basic protein (MBP) is one of the key autoantigens in autoimmune neurodegeneration and multiple sclerosis particularly. MBP is highly positively charged and lacks distinct structure in solution and therefore its intracellular part-ners are still mostly enigmatic. Here we used combination of formaldehyde-induced cross-linking followed by immunoprecipitation and Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) to elucidate the interaction network of MBP in mammalian cells and provide the list of potential MBP interacting proteins. Our data suggest that the largest group of MBP-interacting proteins belongs to cellular proteins involved in the protein translation machinery, as well as in the spatial and temporal regulation of translation. MBP interacts with core ribosomal proteins, RNA helicase Ddx28 and RNA-binding proteins STAU1, ADAR-1 and hnRNP A0, which are involved in various stages of RNA biogenesis and processing, including specific maintaining MBP-coding mRNA. Among MBP partners we identified CTNND1, which has previously been shown to be necessary for myelinating Schwann cells, for cell-cell interactions and the formation of a normal myelin sheath. MBP binds proteins MAGEB2/D2 associated with neurotrophin receptor p75NTR, involved in pathways that promote neuronal survival and neuronal death. Finally, we observed that MBP interacts with RNF40 – a component of heterotetrameric Rnf40/Rnf20 E3 ligase complex, recruited by Egr2, which is the central transcriptional regulator of peripheral myelination. Concluding, our data suggest that MBP may be more actively involved in myelination not only as a main building block but also as a regulating element.
HostingRepository	PRIDE
AnnounceDate	2022-02-16
AnnouncementXML	Submission_2022-02-16_10:40:44.508.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD028685
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Georgij Arapidi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-21 10:27:33	ID requested
⏵ 1	2022-02-16 10:40:45	announced

Smirnova EV, Rakitina TV, Ziganshin RH, Arapidi GP, Saratov GA, Kudriaeva AA, Belogurov AA, Comprehensive Atlas of the Myelin Basic Protein Interaction Landscape. Biomolecules, 11(11):(2021) [pubmed]

submitter keyword: Myelin basic protein, protein-protein interaction, formaldehyde-induced cross-linking, Liquid Chromatography-Tandem Mass Spectrometry

Georgij Pavlovich Arapidi
contact affiliation	Laboratory of olecular oncology, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry
contact email	arapidi@gmail.com
lab head
Georgij Arapidi
contact affiliation	Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency
contact email	arapidi@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD028685
     1. Label: PRIDE project
     2. Name: Comprehensive atlas of the myelin basic protein interaction landscape