PXD028681

PXD028681 is an original dataset announced via ProteomeXchange.

Title	Redox Proteomics Analysis of Auranofin Exposed Ovarian Cancer Cells
Description	The effects of Auranofin (AF) on protein expression and protein oxidation in A2780 cancer cells were explored through a robust investigative strategy based on joint expression proteomics and redox proteomics determinations. Bioinformatics analysis of the proteomics data supports the view that the most critical cellular changes elicited by AF treatment consist of thioredoxin reductase inhibition, alteration of the cell redox state, impairment of the mitochondrial functions, associated metabolic changes with conversion to a glycolytic phenotype, induction of ER stress. The occurrence of the above cellular changes was extensively validated by performing direct biochemical assays. Our data are consistent with the concept that AF produces its effects through a multitarget mechanism that mainly affects the redox metabolism and mitochondrial functions and results in severe ER stress. Results are discussed in the frame of the current mechanistic knowledge existing on AF.
HostingRepository	PRIDE
AnnounceDate	2022-05-19
AnnouncementXML	Submission_2022-05-19_13:56:38.400.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Giovanni Chiappetta
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-21 10:11:18	ID requested
⏵ 1	2022-05-19 13:56:39	announced

Chiappetta G, Gamberi T, Faienza F, Limaj X, Rizza S, Messori L, Filomeni G, Modesti A, Vinh J, Redox proteome analysis of auranofin exposed ovarian cancer cells (A2780). Redox Biol, 52():102294(2022) [pubmed]

submitter keyword: redox proteomics, cysteine, Auranofin, gold, ovarian cancer

Joelle Vinh
contact affiliation	Biological Mass Spectrometry and Proteomics Group, SMBP, PDC UMR 8249 CNRS, ESPCI Paris, Université PSL, 10 rue Vauquelin 75005, Paris, France
contact email	joelle.vinh@espci.fr
lab head
Giovanni Chiappetta
contact affiliation	ESPCI-CNRS
contact email	giovanni.chiappetta@espci.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/05/PXD028681

PRIDE project URI

• PRIDE
  1. PXD028681
     1. Label: PRIDE project
     2. Name: Redox Proteomics Analysis of Auranofin Exposed Ovarian Cancer Cells