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PXD028675

PXD028675 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMurine keratinocytes NFAT5 knockout secretome LC-MS/MS
DescriptionThe skin protects the human body against dehydration and harmful challenges. Keratinocytes (KCs) are the most frequent epidermal cells, and it is anticipated that KC-mediated transport of Na+ ions creates a physiological barrier of high osmolality against the external environment. We studied in KCs the role of NFAT5, a transcription factor whose activity is controlled by osmotic stress. Cultured KCs from adult mice secrete more than 300 proteins, and upon NFAT5 ablation, the secretion of several matrix proteinases, including metalloproteinase-3 (Mmp3) and kallikrein-related peptidase 7 (Klk7), was markedly enhanced. An increase in Mmp3 and Klk7 RNA levels was also detected in transcriptomes of Nfat5-/- KCs, along with increases of numerous components of ‘Epidermal Differentiation Complex’ (EDC), as proline-rich Sprr and S100 proteins. NFAT5 and Mmp3 are co-expressed in basal KCs from fetal and adult skin but not in skin of newborn mice. This is correlated with a strong increase in Mmp3 and Klk7 expression in KCs of newborn mice and suggests, along with the fragile epidermis of adult Nfat5-/- mice, a suppressive effect of NFAT5 on the expression of matrix proteases in skin. Our data suggest that NFAT5 controls the expression of matrix proteases in skin and contributes to the many fold changes during embryonal skin development and skin integrity in adults.
HostingRepositoryPRIDE
AnnounceDate2021-11-16
AnnouncementXMLSubmission_2021-11-16_06:38:10.214.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMalte Sielaff
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListiodoacetamide derivatized residue
InstrumentSynapt MS
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-09-21 09:02:48ID requested
12021-11-16 06:38:10announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: mouse, mus musculus, keratinocyte, NFAT5, secretome, LC-MS/MS, UDMSE, LFQ
Contact List
Stefan Tenzer
contact affiliationInstitute for Immunology University Medical Center of the Johannes Gutenberg-University Mainz 55131 Mainz, Germany
contact emailtenzer@uni-mainz.de
lab head
Malte Sielaff
contact affiliationUniversity Medical Center Mainz
contact emailmalte.sielaff@uni-mainz.de
dataset submitter
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Dataset FTP location
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