PXD028672

PXD028672 is an original dataset announced via ProteomeXchange.

Title	Deciphering BTH-induced response of tomato (Solanum lycopersicum L) and its influence on plant virus infection through a multi-omics approach
Description	The plant response may be triggered by various factors, including pathogens, non-pathogenic microbes, and natural or synthetic molecules, such as salicylic acid (SA), benzo(1,2,3)-Thiadiazole-7-Carbothioic Acid S-Methyl Ester (BTH) that are considered as plant resistance inducers. Resistance inducers mobilize the plant for the synthesis of defense compounds, but they are not directly toxic for the plant. The BTH is an analogue of SA, a molecule naturally synthesized in the plant during systemic acquired resistance (SAR). In this study, we hypothesized that challenging the plant with resistance inducer causes an increase in the level of proteins associated with defence response to external stimuli with primary metabolism and that the action of the choline derivative of the BTH (in the form of the ionic liquid) during induction of resistance in tomato plants is mediated by to some extent similar signaling pathways as it is in the case of unmodified BTH compound. Therefore, this study aimed to identify global proteome changes occurring in tomato plants exposed to resistance inducers as well as to identify common pathways associated with the plant's defense response to (+)ssRNA viruses induced upon both, viral infection and prior resistance inducer treatment. The effect of ionic liquids on changes in plant proteome is not described in the literature. The ionic character of compounds offers a great advantage of using them as the plant resistance inducer. The high chemical and thermal stability, as well as low volatility, guarantees safe application in fields. For that reason, comparing the action of the resistance inducer in the form of an ionic liquid with the core compound (BTH) will help to elucidate the mode of action of ionic liquid derivatives in plant resistance induction. To this end, two RNA-type viruses from different families were tested: tomato mosaic virus (ToMV, Virgaviridae) and potato virus Y (PVY, Potyviridae). The most affected processes presented in the results were: photosynthesis, regulation of oxidative stress, metabolism of glutathione and chitin, cell wall reorganization. The increased impact on the regulation of primary metabolism in inducer-treated plants (both for BTH and cholinium ionic liquid derivative). The response to ToMV and PVY was more intense when infected plants were pre-treated with inducers. The increased abundances of defense priming proteins with changes in cell wall organization may block virus transport from infected spots to other tissue of the host plant so that the losses caused by the disease will be lower.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:35:44.380.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Antje Dittmann
SpeciesList	scientific name: Solanum lycopersicum; NCBI TaxID: 4081;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-21 04:21:29	ID requested
1	2022-09-14 11:26:51	announced
⏵ 2	2023-11-14 08:35:44	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: Orbitrap Fusion Tribrid, viral Resistance, leaves, BTH, Solanum lycopersicon,Tomato, PVY, ToMV

Aleksandra Obrepalska-Steplowska
contact affiliation	Department of Molecular Biology and Biotechnology, Institute of Plant Protection—National Research Institute, Poznan, Poland
contact email	ao.steplowska@iorpib.poznan.pl
lab head
Antje Dittmann
contact affiliation	ETH
contact email	antje.dittmann@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/09/PXD028672

PRIDE project URI

• PRIDE
  1. PXD028672
     1. Label: PRIDE project
     2. Name: Deciphering BTH-induced response of tomato (Solanum lycopersicum L) and its influence on plant virus infection through a multi-omics approach