PXD028670

PXD028670 is an original dataset announced via ProteomeXchange.

Title	Sister chromatid exchanges independent of canonical homologous recombination factors.
Description	Sister chromatid exchanges (SCEs) are a product of joint DNA molecules resolution, and are considered to require homologous recombination (HR). Canonical HR factors BRCA1, BRCA2 and RAD51 were indeed essential for SCE induction in response to irradiation-induced DNA breaks. By contrast, replication-blocking agents, including PARP inhibitors, induced SCEs independently of BRCA1, BRCA2 or RAD51. HR-independent SCEs were associated with incomplete DNA replication, as evidenced by post-replicative single-stranded DNA (ssDNA) accumulation and enrichment of PARP inhibitor-induced SCEs at common fragile sites (CFSs). Importantly, PARP-induced DNA lesions were transmitted into mitosis, pointing towards SCEs originating from mitotic processing of underreplicated DNA. We found polymerase theta to be associated to mitotic DNA lesions, to be required for SCE formation and to prevent chromosome fragmentation upon PARP inhibition in HR-defective cells. Combined, our data show that replication-blocking agents lead to underreplicated DNA in mitosis, which is processed into SCEs independently of canonical HR factors.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:28:47.883.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Anastasia Audrey
SpeciesList	scientific name: Xenopus laevis (African clawed frog); NCBI TaxID: 8355;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-21 00:08:26	ID requested
1	2023-03-10 17:32:11	announced
⏵ 2	2023-11-14 08:28:48	announced	2023-11-14: Updated project metadata.

Heijink AM, Stok C, Porubsky D, Manolika EM, de Kanter JK, Kok YP, Everts M, de Boer HR, Audrey A, Bakker FJ, Wierenga E, Tijsterman M, Guryev V, Spierings DCJ, Knipscheer P, van Boxtel R, Ray Chaudhuri A, Lansdorp PM, van Vugt MATM, Sister chromatid exchanges induced by perturbed replication can form independently of BRCA1, BRCA2 and RAD51. Nat Commun, 13(1):6722(2022) [pubmed]

submitter keyword: cross-over, interphase,Xenopus laevis, MS, mitosis, HR, SCE, synthetic lethality, PARP inhibitors, genome stability, biotin

Marcel A.T.M. van Vugt
contact affiliation	Department of Medical Oncology, University Medical Center Groningen, University of Groningen, the Netherlands, Hanzeplein 1, 9713GZ, Groningen, the Netherlands
contact email	m.vugt@umcg.nl
lab head
Anastasia Audrey
contact affiliation	University Medical Center Groningen
contact email	a.audrey@umcg.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD028670

PRIDE project URI

• PRIDE
  1. PXD028670
     1. Label: PRIDE project
     2. Name: Sister chromatid exchanges independent of canonical homologous recombination factors.