PXD028669

PXD028669 is an original dataset announced via ProteomeXchange.

Title	Ubiquitin proteomics reveals critical targets of the Nse1 RING domain in rDNA and genome stability
Description	Ubiquitination controls most cellular processes, and its deregulation is associated to many pathologies. The Nse1 subunit in the Smc5/6 complex contains a RING domain with ubiquitin E3 ligase activity and important functions in genome integrity. However, Nse1-dependent ubiquitin targets remain largely unknown. Here, we use label-free quantitative proteomics to analyse the nuclear ubiquitinome of nse1 RING mutant cells. Our results show that Nse1 impacts on the ubiquitination of several proteins involved in DNA damage tolerance, ribosome biogenesis and metabolism that, importantly, extend beyond canonical functions of the Smc5/6 complex in chromosome segregation. In addition, our analysis uncovers an unexpected connection between Nse1 and Pol I ubiquitination. Specifically, Nse1 promotes the ubiquitination of K408 and K410 in Rpa190, the largest subunit of Pol I, to induce its degradation. We propose that this mechanism contributes to Smc5/6-dependent rDNA disjunction in response to transcriptional elongation defects.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:53:27.204.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pilar Ximenez-Embun
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	ubiquitination signature dipeptidyl lysine; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-21 00:03:56	ID requested
1	2023-05-05 00:19:32	announced
⏵ 2	2023-11-14 08:53:28	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: Nse1, ubiquitination

Javier Muñoz
contact affiliation	Spanish National Cancer Research Centre (CNIO) Address: Melchor Férnandez Almagro, 3. 28029 Madrid. SPAIN Phone: +34 917 328 000 Ext. 3110
contact email	jmunozpe@cnio.es
lab head
Pilar Ximenez-Embun
contact affiliation	Proteomics Group
contact email	mpximenez@cnio.es
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD028669
     1. Label: PRIDE project
     2. Name: Ubiquitin proteomics reveals critical targets of the Nse1 RING domain in rDNA and genome stability