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PXD028661

PXD028661 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe NuRD complex is required for Thpok-mediated CD4+ T cell development
DescriptionThis data represent characterization of the interactome of murine Thpok. A version of Thpok (Thpok-Bio) subject to biotinylation in vivo by the BirA biotin ligase was used; to assess Thpok interactions in primary cells, Thpok-Bio was retrovirally expressed in in vitro activated CD4+ T cells from Thpokfl/fl Ox40-Cre+ mice carrying a Rosa26BirA allele. Expression of Ox40-Cre, initiated upon T cell activation, causes the deletion of endogenous Thpokfl alleles, so that transduced cells only express Thpok-Bio. Two Thpok deletion constructs (BKK and RFK) were also analyzed. Following streptavidin pulldown, proteins were separated by SDS-PAGE, the full lane cut into 9 slices, and in-gel digested. FIles are: 1384 - empty vector; 1385 - Thpok; 1386 - Thpok + Ethidium Bromide; 1387 - Thpok BKK; 1388 - Thpok RFK.
HostingRepositoryMassIVE
AnnounceDate2022-11-15
AnnouncementXMLSubmission_2022-11-15_07:07:25.283.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterLisa Jenkins
SpeciesList scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090;
ModificationListOxidation; Carbamidomethyl
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-09-20 10:20:59ID requested
12022-11-15 07:07:25announced
Publication List
no publication
Keyword List
submitter keyword: Thpok, Interactome
Contact List
Lisa Jenkins
contact affiliationNCI, NIH
contact emailjenkinsl@mail.nih.gov
lab head
Lisa Jenkins
contact affiliationNCI
contact emailjenkinsl@mail.nih.gov
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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