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PXD028647

PXD028647 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA shot to missing protein from an aneuploidy cell line
DescriptionWith fast development of proteomic technology, the scale of missing proteins (MPs) has being continuously shrunk, approximately 1,470 MPs not explored yet. Discovery of MPs, on the other hand, is appearing more difficult. In some aneuploid cells, the abundance changes are proportional to the additional chromosome(s), while several MPs were found within them. Herein, we hypothesize that a stable aneuploid cell line with the increased chromosomes was a useful material that assists MP exploration. Ker-CT cell line with trisomy at chromosome 5 and 20 was selected. With a combination strategy of RNA-Seq and LC-M/MS, a total of 22,178 transcripts and 8,846 proteins were identified in Ker-CT. Although the transcripts corresponding to 31 and 32 MP genes located at chromosome 5 and 20 were detected, none of MPs was found in Ker-CT. Surprisingly, 3 MPs containing at least two unique non-nest peptides of length ≥9 amino acid were identified in Ker-CT, whose genes are located in chromosome 3 and 10. Furthermore, the 3 MPs were verified using the method of parallel reaction monitoring (PRM).
HostingRepositoryPRIDE
AnnounceDate2021-11-25
AnnouncementXMLSubmission_2021-11-25_07:07:20.411.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterBu fanyu
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-09-20 02:50:53ID requested
12021-11-25 07:07:20announced
Publication List
Dataset with its publication pending
Keyword List
ProteomeXchange project tag: Chromosome-centric Human Proteome Project (C-HPP), Human Proteome Project
submitter keyword: Missing proteins, RNA-Seq, LC-MS/MS, Karyotype, Aneuploidy
Contact List
Liu Si Qi
contact affiliationBGI-Genomics and Department of BGI education, School of Life Sciences, University of Chinese Academy of Science, China
contact emailsiqiliu@genomics.cn
lab head
Bu fanyu
contact affiliation15105131523
contact emailbufanyu@genomics.cn
dataset submitter
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Dataset FTP location
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