PXD028647 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A shot to missing protein from an aneuploidy cell line |
Description | With fast development of proteomic technology, the scale of missing proteins (MPs) has being continuously shrunk, approximately 1,470 MPs not explored yet. Discovery of MPs, on the other hand, is appearing more difficult. In some aneuploid cells, the abundance changes are proportional to the additional chromosome(s), while several MPs were found within them. Herein, we hypothesize that a stable aneuploid cell line with the increased chromosomes was a useful material that assists MP exploration. Ker-CT cell line with trisomy at chromosome 5 and 20 was selected. With a combination strategy of RNA-Seq and LC-M/MS, a total of 22,178 transcripts and 8,846 proteins were identified in Ker-CT. Although the transcripts corresponding to 31 and 32 MP genes located at chromosome 5 and 20 were detected, none of MPs was found in Ker-CT. Surprisingly, 3 MPs containing at least two unique non-nest peptides of length ≥9 amino acid were identified in Ker-CT, whose genes are located in chromosome 3 and 10. Furthermore, the 3 MPs were verified using the method of parallel reaction monitoring (PRM). |
HostingRepository | PRIDE |
AnnounceDate | 2021-11-25 |
AnnouncementXML | Submission_2021-11-25_07:07:20.411.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Bu fanyu |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-09-20 02:50:53 | ID requested | |
⏵ 1 | 2021-11-25 07:07:20 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
ProteomeXchange project tag: Chromosome-centric Human Proteome Project (C-HPP), Human Proteome Project |
submitter keyword: Missing proteins, RNA-Seq, LC-MS/MS, Karyotype, Aneuploidy |
Contact List
Liu Si Qi |
contact affiliation | BGI-Genomics and Department of BGI education, School of Life Sciences, University of Chinese Academy of Science, China |
contact email | siqiliu@genomics.cn |
lab head | |
Bu fanyu |
contact affiliation | 15105131523 |
contact email | bufanyu@genomics.cn |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD028647
- Label: PRIDE project
- Name: A shot to missing protein from an aneuploidy cell line