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PXD028646

PXD028646 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleEpstein-Barr Virus+ B Cells in the Breast Cancer Immune Response: A Case Report.
DescriptionTumor-Infiltrating Lymphocytes (TILs) are composed by several immune subpopulations including NK, NKT, Tab, Tgd and B cells. Although some of these cells can have antitumoral activities, there is also a large number of cells which are not tumor-specific,known as bystander cells. These cells may not have a direct role in the antitumoral response but could lead to a modulation of the immune response after time. Recent studies have described fatal encephalitis and myocarditis in cancer patients after immune checkpoint therapies, in which EBV-specific T cells may be involved. Since EBV is able to infect B cells and epithelial cells, both present in the primary tumor site, the first events of these fatal responses could be a consequence of a dysregulation of the immune system in the tumor microenvironment, although this have not been deeply studied. Thus, the main objectives of this study are (i) to use EBV-transformed B cells from the tumor site as a model to study the tumor-infiltrating EBV+ B cells and (ii) to use these EBV-derived B cells to study the relationship with autologous T cells isolated from the tumor site. T cells from TILs have been isolated from a triple negative breast cancer patient before neoadjuvancy and were expanded directly from the biopsy. A Patient-Derived Xenograft (PDX) generated from the breast tumor, progressed into a lymphocytic neoplasm. EBVtransformed B cells were obtained from this PDX (PDX-derived B cells). T Cell Receptor high-throughput sequencing has been used to monitor the clonotypes present in the different samples. We have found 43 clonotypes infiltrating the PDX-562 tissue and 18 of them were also present in the human biopsy or in the biopsy-derived T cell cultures. 5 of these sequences were found in one of the biopsy-derived T cells analyzed. Thus, PDXderived B cells have been used as antigen presenting cells to expand these cells. Few clonotypes were obtained after expansions, although the analysis of the middle CDR3 have revealed a similar pattern with the 18 sequences from the PDX sample. Furthermore, peptides presented by HLA have been eluted from PDX-derived B cells, followed by MS analysis. Peptides obtained derived from 31 different proteins and >40% are cancerrelated in terms of expression.
HostingRepositoryPRIDE
AnnounceDate2021-11-17
AnnouncementXMLSubmission_2021-11-17_06:35:51.484.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMontserrat Carrascal
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-09-20 02:50:21ID requested
12021-11-17 06:35:51announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: tumor-infiltrating EBV+ B cells, immunopeptidomics, TCR, cancer, TILs
Contact List
Mercè Martí
contact affiliationInstitut de Biotecnologia i Biomedicina, Universitat Autònoma de Barcelona, Cerdanyola del Vallés, Barcelona, Spain.
contact emailMerce.Marti@uab.cat
lab head
Montserrat Carrascal
contact affiliationIIBB-CSIC
contact emailmontserrat.carrascal.csic@uab.cat
dataset submitter
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