PXD028639

PXD028639 is an original dataset announced via ProteomeXchange.

Title	Identification of differentially expressed proteins in human pluripotent stem cell derived into dopaminergic neurons
Description	Dopaminergic neurons located in the ventral midbrain can be broadly subdivided into two distinct subpopulations. Substantia nigra (SN) dopaminergic neurons are highly sensitive to toxic insults and selectively degenerate in Parkinson’s disease, while ventral tegmental area (VTA) dopaminergic neurons are associated with other neurological disorders. Access to enriched cultures of SN and VTA dopaminergic neuronal subpopulations will facilitate disease modelling and give insight in the differential vulnerability, but it is unclear how the differentiation of human ES cells can be directed towards these distinct lineages. We found that overexpression of the lineage specifying transcription factors Sox6 and Otx2 can direct the differentiation of human ES cells into enriched populations of respectively SN or VTA neurons. Proteomic analysis of these cultures resulted in the identification of several differential expressed proteins and provided insight in pathways contributing to the selective vulnerability of SN.
HostingRepository	PRIDE
AnnounceDate	2021-11-03
AnnouncementXML	Submission_2021-11-03_08:01:25.395.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD028639
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Valerie LABAS
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-19 22:41:27	ID requested
⏵ 1	2021-11-03 08:01:25	announced

Dataset with its publication pending

submitter keyword: pluripotent stem cells, dopaninergic neurons, Parkinson disease

Lia Panman
contact affiliation	MRC toxicology unit Hodgkin building Lancaster road LE1 9HN Leicester United Kingdom
contact email	lp213@leicester.ac.uk
lab head
Valerie LABAS
contact affiliation	INRAE
contact email	valerie.labas@inrae.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD028639
     1. Label: PRIDE project
     2. Name: Identification of differentially expressed proteins in human pluripotent stem cell derived into dopaminergic neurons