PXD028637

PXD028637 is an original dataset announced via ProteomeXchange.

Title	RPA promotes processive ssDNA digestion by Dna2 via their bimodal interaction in Saccharomyces cerevisiae
Description	Single-stranded DNA (ssDNA) widely exists as intermediates in DNA metabolic pathways. The ssDNA binding protein, RPA, not only protects the integrity of ssDNA, but also directs the downstream factor that signals or repairs the ssDNA intermediate. However, it remains unclear how these enzymes/factors out-compete RPA and access to ssDNA. Using the budding yeast, Saccharomyces cerevisiae, as a model system, we discovered that Dna2, a key nuclease in DNA replication and repair, employs a bimodal interface to act with RPA both in cis and in trans. The cis-action renders RPA a processive unit for Dna2-catalyzed ssDNA digestion, where RPA actively delivers its bound ssDNA to Dna2. The trans-action mediated by an acidic patch from Dna2, on the other hand, enables Dna2 to operatie with a sub-optimal amount of RPA or to overcome DNA secondary structures. Genetically, this trans-action mode is not required for cell viability, but indispensable for successful DSB repair.
HostingRepository	PRIDE
AnnounceDate	2022-02-22
AnnouncementXML	Submission_2022-02-22_04:22:32.803.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yixiang Zhang
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	monohydroxylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-19 22:22:06	ID requested
⏵ 1	2022-02-22 04:22:33	announced

Shen J, Zhao Y, Pham NT, Li Y, Zhang Y, Trinidad J, Ira G, Qi Z, Niu H, Deciphering the mechanism of processive ssDNA digestion by the Dna2-RPA ensemble. Nat Commun, 13(1):359(2022) [pubmed]

submitter keyword: Dna2, RPA, ssDNA, Saccharomyces cerevisiae

Hengyao Niu
contact affiliation	Department of Molecular and Cellular Biochemistry, Indiana University, Bloomington, IN, 47405
contact email	hniu@indiana.edu
lab head
Yixiang Zhang
contact affiliation	Indiana University
contact email	alexzhangyx@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD028637
     1. Label: PRIDE project
     2. Name: RPA promotes processive ssDNA digestion by Dna2 via their bimodal interaction in Saccharomyces cerevisiae