PXD028620

PXD028620 is an original dataset announced via ProteomeXchange.

Title	Proteomics insights into the molecular mechanisms of c9, t11-CLA synthesis
Description	Cis-9, trans-11-conjugated linoleic acid (c9, t11-CLA), a functional fatty acid, is one of the research hotspots in the fields of functional dairy products development because of its multiple beneficial effects in humans and animals. In milk, most c9, t11-CLA is de novo synthesized from trans-11-octadecenoic acid (TVA) and it is confirmed that genes such as stearoyl-coA desaturase 1 (SCD1) play a key role in the synthesis. However, few studies have been reported to deeply elucidate the SCD1-dependent molecular mechanism of cis9, trans11-CLA synthesis and its relationship with the pathways of energy metabolism and lipid metabolism. Therefore, in the present study, MAC-T cells were divided into three groups: CAY group (SCD1-inhibited MAC-T cell model with the addition of CAY, TVA), TVA group (only TVA), and Control group (without CAY, TVA). The relative mRNA expression of SCD1 was measured using real-time PCR, while TVA accumulation and c9, t11 -CLA synthesis were analyzed using gas chromatography (GC). The SCD1-related proteins were firstly screened in MAC-T cells by Tandem Mass Tag (TMT)-based quantitative proteomics analysis, then their functions were annotated by bioinformatic analysis, and finally their relationships with SCD1 were evaluated by parallel reaction monitoring (PRM) analysis and small RNA interference. The results showed that the deficiency of SCD1 led by CAY10566 blocked the synthesis of c9, t11-CLA in MAC-T cells. Sixty-one SCD1-associated proteins were screened and found to be mainly involved in the pathways of energy metabolism and lipid metabolism, such as glycolytic pathway, pentose phosphate pathway, fatty acid elongation pathway, and unsaturated fatty acid biosynthesis. Among these genes, 17 proteins were validated under the PRM analysis. PGAM1 (phosphoglycerate mutase 1), TPI1 (triosephosphate isomerase 1), LDHB (lactate dehydrogenase B), and ALDOA (aldolase, fructose-bisphosphate A) were verified to have a negative relationships with SCD1. This study furthered our understanding of the molecular mechanisms of c9, t11-CLA synthesis in the mammary glands of dairy cows.
HostingRepository	PRIDE
AnnounceDate	2022-08-12
AnnouncementXML	Submission_2022-08-11_22:29:35.292.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	TAO WANG
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-17 09:11:20	ID requested
⏵ 1	2022-08-11 22:29:36	announced

Peng L, Bai G, Wang C, Dong J, Liu Y, Sun Z, Zhen Y, Qin G, Zhang X, Demelash N, Wang T, 11-Conjugated Linoleic Acid Biosynthesis in Bovine Mammary Gland Epithelial Cells. Animals (Basel), 12(13):(2022) [pubmed]

submitter keyword: Cis-9, trans-11-conjugated linoleic acid

Stearoyl-CoA desaturase 1

MAC-T cells

Proteomics

Functional fatty acid

Tao Wang
contact affiliation	College of Animal Science and Technology, Key Laboratory of Animal Nutrition and Feed Science of Jilin Province, Key Laboratory of Animal Production Product Quality and Security Ministry of Education, Jilin Agricultural University, China
contact email	cagewang@163.com
lab head
TAO WANG
contact affiliation	Jilin Agricultural University
contact email	cagewang@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD028620
     1. Label: PRIDE project
     2. Name: Proteomics insights into the molecular mechanisms of c9, t11-CLA synthesis