PXD028619

PXD028619 is an original dataset announced via ProteomeXchange.

Title	MacAB-TolC, a determinant of solid-liquid biofilms in Acinetobacter baumannii
Description	Acinetobacter baumannii has emerged as one of the most problematic opportunist bacterial pathogen responsible for hospital-acquired and community infections worldwide. Besides its high capacities to acquire antibiotic resistance mechanisms, it also presents high adhesion abilities on inert and living surfaces leading to biofilm development, a lifestyle conferring an additional protection against various treatments, and allowing it to persist for long periods in various hospital niches. Due to their increasing resilience to antimicrobial treatments, A. baumannii biofilms are difficult to control and ultimately eradicate. Overcoming the significant challenges pose by A. baumannii require fundamental insights into mechanisms involved in the development of mature biofilm and thus, may help to develop novel strategies for biofilm prevention and control. To unravel critical determinants of this sessile lifestyle, we compared the proteome profiles of bacteria grown in planktonic stationary phase with those of two A. baumannii strains (ATCC 17978 and SDF), harboring specific features regarding biofilm formation, grown in mature solid-liquid (S-L) biofilm using a proteomic quantitative study. Of interest, among the 69 common proteins determinants accumulated in the two strains at the S-L interface, we sorted out the MacAB-TolC system. This tripartite efflux pump appears to play a role in A. baumannii biofilm formation as demonstrated by using ΔmacAB-tolC deletion mutant. Complementary approaches allowed us to get an overview of the impact of macAB-tolC deletion in A. baumannii physiology. Indeed, this efflux pump appeared to be involved in the envelope stress response occurring in mature biofilm and contributes to maintain WT membrane rigidity as well as tolerance to high osmolarity conditions. In addition, this system is probably involved in the maintenance of iron and sulfur homeostasis. MacAB-TolC might help this pathogen facing and adapting to biofilm architecture which is heterogeneous in space and time, especially in mature biofilm. Increasing our knowledge of A. baumannii biofilm formation will undoubtedly help us develop new therapeutic strategies to tackle this emerging threat to human health.
HostingRepository	PRIDE
AnnounceDate	2022-02-16
AnnouncementXML	Submission_2022-02-16_10:27:17.305.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Julie Hardouin
SpeciesList	scientific name: Acinetobacter baumannii; NCBI TaxID: 470;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-17 09:11:20	ID requested
⏵ 1	2022-02-16 10:27:18	announced

Robin B, Nicol M, Le H, Tahrioui A, Schaumann A, Vuillemenot JB, Vergoz D, Lesouhaitier O, Jouenne T, Hardouin J, Potron A, Perrot V, D, é E, Biofilm at the Solid-Liquid Interface. Front Microbiol, 12():785161(2021) [pubmed]

submitter keyword: solid-liquid interface, biofilm, efflux pump, eDNA, envelop stress response

Emmanuelle Dé
contact affiliation	Equipe BRICS, Laboratoire PBS UMR 6270 CNRS, Université de Rouen
contact email	emmanuelle.de@univ-rouen.fr
lab head
Julie Hardouin
contact affiliation	Université de Rouen - laboratoire PBS
contact email	julie.hardouin@univ-rouen.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD028619
     1. Label: PRIDE project
     2. Name: MacAB-TolC, a determinant of solid-liquid biofilms in Acinetobacter baumannii