PXD028619 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | MacAB-TolC, a determinant of solid-liquid biofilms in Acinetobacter baumannii |
Description | Acinetobacter baumannii has emerged as one of the most problematic opportunist bacterial pathogen responsible for hospital-acquired and community infections worldwide. Besides its high capacities to acquire antibiotic resistance mechanisms, it also presents high adhesion abilities on inert and living surfaces leading to biofilm development, a lifestyle conferring an additional protection against various treatments, and allowing it to persist for long periods in various hospital niches. Due to their increasing resilience to antimicrobial treatments, A. baumannii biofilms are difficult to control and ultimately eradicate. Overcoming the significant challenges pose by A. baumannii require fundamental insights into mechanisms involved in the development of mature biofilm and thus, may help to develop novel strategies for biofilm prevention and control. To unravel critical determinants of this sessile lifestyle, we compared the proteome profiles of bacteria grown in planktonic stationary phase with those of two A. baumannii strains (ATCC 17978 and SDF), harboring specific features regarding biofilm formation, grown in mature solid-liquid (S-L) biofilm using a proteomic quantitative study. Of interest, among the 69 common proteins determinants accumulated in the two strains at the S-L interface, we sorted out the MacAB-TolC system. This tripartite efflux pump appears to play a role in A. baumannii biofilm formation as demonstrated by using ΔmacAB-tolC deletion mutant. Complementary approaches allowed us to get an overview of the impact of macAB-tolC deletion in A. baumannii physiology. Indeed, this efflux pump appeared to be involved in the envelope stress response occurring in mature biofilm and contributes to maintain WT membrane rigidity as well as tolerance to high osmolarity conditions. In addition, this system is probably involved in the maintenance of iron and sulfur homeostasis. MacAB-TolC might help this pathogen facing and adapting to biofilm architecture which is heterogeneous in space and time, especially in mature biofilm. Increasing our knowledge of A. baumannii biofilm formation will undoubtedly help us develop new therapeutic strategies to tackle this emerging threat to human health. |
HostingRepository | PRIDE |
AnnounceDate | 2022-02-16 |
AnnouncementXML | Submission_2022-02-16_10:27:17.305.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Julie Hardouin |
SpeciesList | scientific name: Acinetobacter baumannii; NCBI TaxID: 470; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-09-17 09:11:20 | ID requested | |
⏵ 1 | 2022-02-16 10:27:18 | announced | |
Publication List
Robin B, Nicol M, Le H, Tahrioui A, Schaumann A, Vuillemenot JB, Vergoz D, Lesouhaitier O, Jouenne T, Hardouin J, Potron A, Perrot V, D, é E, Biofilm at the Solid-Liquid Interface. Front Microbiol, 12():785161(2021) [pubmed] |
Keyword List
submitter keyword: solid-liquid interface, biofilm, efflux pump, eDNA, envelop stress response |
Contact List
Emmanuelle Dé |
contact affiliation | Equipe BRICS, Laboratoire PBS UMR 6270 CNRS, Université de Rouen |
contact email | emmanuelle.de@univ-rouen.fr |
lab head | |
Julie Hardouin |
contact affiliation | Université de Rouen - laboratoire PBS |
contact email | julie.hardouin@univ-rouen.fr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD028619
- Label: PRIDE project
- Name: MacAB-TolC, a determinant of solid-liquid biofilms in Acinetobacter baumannii