PXD028605

PXD028605 is an original dataset announced via ProteomeXchange.

Title	Human whole blood processed with VAMS device
Description	This project tested the use of a volumemetric absorptive microsampling device as a substrate to prepare whole blood for proteomic analysis by LC-MS. We demonstrated that VAMS are a useful substrate to trap proteins from blood, wash them to reduce highly abundant protein species which enabled single shot LC-MS. This led to the detection of more than 1600 proteins in a single run. We further tested different washing reagents and compared VAMS against paper DBS. Finally, we demonstrated an application of using VAMS to prepare archival, frozen whole blood cell pellets from non-small cell lung cancer patients for biomarker studies.
HostingRepository	PRIDE
AnnounceDate	2022-02-21
AnnouncementXML	Submission_2022-02-21_11:09:24.145.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mark Molloy
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-16 23:55:15	ID requested
⏵ 1	2022-02-21 11:09:24	announced

Molloy MP, Hill C, O'Rourke MB, Chandra J, Steffen P, McKay MJ, Pascovici D, Herbert BR, Proteomic Analysis of Whole Blood Using Volumetric Absorptive Microsampling for Precision Medicine Biomarker Studies. J Proteome Res, 21(4):1196-1203(2022) [pubmed]

submitter keyword: human, blood, plasma, LC-MS, VAMS, microsampling

Mark P Molloy
contact affiliation	Bowel Cancer and Biomarker Laboratory School of Medical Sciences The University of Sydney
contact email	m.molloy@sydney.edu.au
lab head
Mark Molloy
contact affiliation	The University of Sydney
contact email	m.molloy@sydney.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD028605

PRIDE project URI

• PRIDE
  1. PXD028605
     1. Label: PRIDE project
     2. Name: Human whole blood processed with VAMS device