PXD028584

PXD028584 is an original dataset announced via ProteomeXchange.

Title	Sarcoglycan interactions in murine muscle membranes
Description	The sarcoglycan complex (SC) is part of a network that links the striated muscle cytoskeleton to the basal lamina across the sarcolemma. The SC coordinates changes in phosphorylation and Ca++-flux during mechanical deformation, and these processes are disrupted with loss-of-function mutations in gamma-sarcoglycan (Sgcg) that cause Limb Girdle Muscular Dystrophy 2C/R5. To gain insight into how the SC mediates mechano-signaling in muscle, we utilized LC-MS/MS proteomics to identify SC-associated proteins in immunoprecipitates from enriched sarcolemmal fractions. Criteria for inclusion were selective co-immunoprecipitation with anti-Sgcg from C57BL/6 control muscle, but under-representation in parallel experiments with Sgcg-null muscle or with non-specific IgG. Co-immunoprecipitation from archvillin-deleted muscle (Svil-null) was also evaluated.
HostingRepository	PRIDE
AnnounceDate	2022-02-16
AnnouncementXML	Submission_2022-02-16_10:04:52.163.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD028584
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Scott Shaffer
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	2-pyrrolidone-5-carboxylic acid (Gln); monohydroxylated residue; mono N-acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-16 09:00:11	ID requested
⏵ 1	2022-02-16 10:04:53	announced

Smith TC, Vasilakos G, Shaffer SA, Puglise JM, Chou CH, Barton ER, Luna EJ, -sarcoglycan interactors in murine muscle membranes. Skelet Muscle, 12(1):2(2022) [pubmed]

submitter keyword: Limb Girdle Muscular Dystrophy, muscle proteomics, skeletal muscle, sarcolemma, sarcoglycan complex, archvillin, protein-protein interactions, immunoaffinity purification, shotgun proteomics, membrane biochemistry

Elizabeth J. Luna
contact affiliation	Department of Radiology, Division of Cell Biology & Imaging, University of Massachusetts Medical School, Worcester, MA, USA
contact email	Elizabeth.Luna@umassmed.edu
lab head
Scott Shaffer
contact affiliation	University of Massachusetts Medical School
contact email	scott.shaffer@umassmed.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD028584

PRIDE project URI

• PRIDE
  1. PXD028584
     1. Label: PRIDE project
     2. Name: Sarcoglycan interactions in murine muscle membranes