PXD028577

PXD028577 is an original dataset announced via ProteomeXchange.

Title	Effect of POR knockdown on the proteome of HepaRG cells
Description	Cytochrome P450 oxidoreductase (POR) is involved in the oxidative metabolism of xenobiotics and endogenous compounds like fatty acid and cholesterol. While effects of diminished Por were extensively studied in mouse models, studies in human models are still missing. We used previously established CRISPR/Cas9-mediated POR knockdown in HepaRG cells as a human hepatic cell model to investigate the effects of POR knockdown on global protein expression levels.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:30:14.858.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thierry Schmidlin
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	6550 iFunnel Q-TOF LC/MS

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-16 08:59:35	ID requested
1	2021-11-04 00:24:58	announced
2	2021-11-30 14:15:55	announced	2021-11-30: Updated publication reference for DOI(s): 10.3389/fphar.2021.769703.
3	2023-11-14 08:53:53	announced	2023-11-14: Updated project metadata.
⏵ 4	2024-10-22 05:30:15	announced	2024-10-22: Updated project metadata.

10.3389/FPHAR.2021.769703;

ProteomeXchange project tag: Liver (B/D-HPP), Biology/Disease-Driven Human Proteome Project (B/D-HPP), Human Proteome Project

submitter keyword: proteomics,HepaRG, bile acid metabolism, CRISPR/Cas9, cholesterol biosynthesis, NADPH cytochrome P450 reductase

Ute Hofmann
contact affiliation	Dr. Margarete Fischer-Bosch Institute of Clinical Pharmacology Auerbachstr. 112 70376 Stuttgart Germany
contact email	Ute.Hofmann@ikp-stuttgart.de
lab head
Thierry Schmidlin
contact affiliation	Oxford University
contact email	thierry.schmidlin@ndm.ox.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/11/PXD028577

PRIDE project URI

• PRIDE
  1. PXD028577
     1. Label: PRIDE project
     2. Name: Effect of POR knockdown on the proteome of HepaRG cells