PXD028564

PXD028564 is an original dataset announced via ProteomeXchange.

Title	Elucidation of the protein composition of mouse seminal vesicle fluid
Description	The seminal vesicles are male accessory sex glands that contribute the bulk of the seminal plasma in which mammalian spermatozoa are bathed during ejaculation. In addition to helping convey sperm through the ejaculatory duct, seminal vesicle secretions support sperm survival after ejaculation and influence the female reproductive tract to promote receptivity to pregnancy. Despite its biological importance, there is limited understanding of seminal vesicle fluid (SVF) composition with only 75 proteins having been identified in publicly available proteomic analyses. To address this limitation, here we report new preparative methodology involving sequential solubilization of mouse SVF in guanidine hydrochloride, prior to acetone precipitation and analysis by label-free quantitative mass spectrometry. This strategy identified 126 proteins, including 83 previously undetected in SVF. Members of the seminal vesicle secretory protein family were the most abundant, accounting for 79% of all peptide spectrum matches. Functional analysis of SVF proteins identified inflammation and formation of the vaginal plug as the two most prominent biological processes. Notable additional processes included modulation of sperm function and regulation of the female reproductive tract immune environment. Together, these findings provide a robust methodological framework for future SVF studies and identify novel proteins with the potential to influence both male and female reproductive health.
HostingRepository	PRIDE
AnnounceDate	2022-01-20
AnnouncementXML	Submission_2022-01-20_15:56:17.268.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD028564
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	David Skerrett-Byrne
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-16 07:40:38	ID requested
⏵ 1	2022-01-20 15:56:18	announced

Smyth SP, Nixon B, Anderson AL, Murray HC, Martin JH, MacDougall LA, Robertson SA, Skerrett-Byrne DA, Schjenken JE, Elucidation of the protein composition of mouse seminal vesicle fluid. Proteomics, 22(9):e2100227(2022) [pubmed]

submitter keyword: bioinformatics, proteomics, seminal vesicle fluid

David Skerrett-Byrne
contact affiliation	Priority Research Centre for Reproductive Science, College of Engineering, Science and Environment, The University of Newcastle, Australia
contact email	David.Skerrett-Byrne@newcastle.edu.au
lab head
David Skerrett-Byrne
contact affiliation	The University of Newcastle
contact email	David.Skerrett-Byrne@newcastle.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD028564
     1. Label: PRIDE project
     2. Name: Elucidation of the protein composition of mouse seminal vesicle fluid