PXD028553

PXD028553 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of the reaction center-light harvesting 1 complex from Rhodobacter sphaeroides, identifying a previously uncharacterized protein component encoded by a newly-identified ORF.
Description	Photosynthesis in purple bacteria, represented by Rhodobacter sphaeroides as a long-established model organism, utilizes a protein-cofactor complex to capture solar energy. This complex is embedded in specialized spherical membrane structures and comprises a central reaction center (RC) encircled by a ring of light harvesting 1 (LH1) polypeptides. An additional protein PufX has two functions: (1) facilitating ubiquinone/ubiquinol exchange between the RC and membrane and (2) inducing complex dimerization – important for maintaining the curvature of the membrane structures. Cryo-EM has revealed a further component that facilitates dimerization, with proteomic analysis confirming its identity as a previously uncharacterized protein encoded by a newly-identified ORF.
HostingRepository	PRIDE
AnnounceDate	2022-02-16
AnnouncementXML	Submission_2022-02-16_09:45:47.274.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Philip Jackson
SpeciesList	scientific name: Rhodobacter sphaeroides 2.4.1; NCBI TaxID: 272943;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-16 07:39:28	ID requested
⏵ 1	2022-02-16 09:45:48	announced

Qian P, Croll TI, Hitchcock A, Jackson PJ, Salisbury JH, Castro-Hartmann P, Sader K, Swainsbury DJK, Hunter CN, : the structural basis for dimerisation. Biochem J, 478(21):3923-3937(2021) [pubmed]

submitter keyword: LC-MS/MS, Rhodobacter sphaeroides, purple bacteria, photosynthesis, reaction center light-harvesting-1- complex, dimerization

Christopher Neil Hunter
contact affiliation	School of Biosciences, University of Sheffield
contact email	c.n.hunter@sheffield.ac.uk
lab head
Philip Jackson
contact affiliation	Department of Molecular Biology and Biotechnology, University of Sheffield
contact email	p.j.jackson@sheffield.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD028553

PRIDE project URI

• PRIDE
  1. PXD028553
     1. Label: PRIDE project
     2. Name: Proteomic analysis of the reaction center-light harvesting 1 complex from Rhodobacter sphaeroides, identifying a previously uncharacterized protein component encoded by a newly-identified ORF.