PXD028551

PXD028551 is an original dataset announced via ProteomeXchange.

Title	Capacitive electrical stimulation effects on osteoblast-released matrix microvesicles
Description	Passive implants currently used in the clinics cannot prevent failure rates and inherent revision arthroplasties. Novel bioelectronic devices that include biophysical stimulators (such as electric stimulators) and sensing systems are desired since these will allow for long-term monitoring and control of the bone-implant interface, in a personalized manner. We have developed acting-sensing dual systems operated at high frequency (HF) that are able to stimulate osteoconduction in pre-osteoblasts and osteoinduction in human adipose-derived mesenchymal stem cells (hASCs). The project included – to our knowledge – the first-time proteomic analysis of microvesicles secreted from osteoblasts electrically-stimulated in vitro by a capacitive stimulator of thin interdigitated electrodes delivering an electrical 60 kHz HF stimulation, 30 min/day. Results revealed regulation of osteodifferentiation and mineralization-related proteins (e.g. Tgfb3, Ttyh3, Itih1, Aldh1a1).
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:52:40.320.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD028551
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Bárbara M de Sousa
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-16 07:39:07	ID requested
1	2021-11-29 06:20:07	announced
2	2021-12-03 01:30:45	announced	2021-12-03: Updated publication reference for DOI(s): 10.1038/s41536-021-00184-6.
3	2021-12-15 07:30:25	announced	2021-12-03: Updated publication reference for DOI(s): 10.1038/s41536-021-00184-6. 2021-12-15: Updated publication reference for PubMed record(s): 34815414. 2021-12-15: Updated publication reference for DOI(s): 10.1038/s41536-021-00184-6.
⏵ 4	2023-11-14 08:52:41	announced	2023-11-14: Updated project metadata.

10.6019/PXD028551;

de Sousa BM, Correia CR, Ferreira JAF, Mano JF, Furlani EP, Soares Dos Santos MP, Vieira SI, Capacitive interdigitated system of high osteoinductive/conductive performance for personalized acting-sensing implants. NPJ Regen Med, 6(1):80(2021) [pubmed]

submitter keyword: extracellular, capacitive coupling, electrical stimulation, bone, osteoblasts, matrix microvesicles, LC-MS/MS

Sandra I. Vieira
contact affiliation	Department of Medical Sciences, Institute of Biomedicine (iBiMED), University of Aveiro, 3810-193 Aveiro, Portugal.
contact email	sivieira@ua.pt
lab head
Bárbara M de Sousa
contact affiliation	Institute of Biomedicine, Department of Medical Sciences, University of Aveiro, Portugal
contact email	bmsousa@ua.pt
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/11/PXD028551

PRIDE project URI

• PRIDE
  1. PXD028551
     1. Label: PRIDE project
     2. Name: Capacitive electrical stimulation effects on osteoblast-released matrix microvesicles