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PXD028551

PXD028551 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCapacitive electrical stimulation effects on osteoblast-released matrix microvesicles
DescriptionPassive implants currently used in the clinics cannot prevent failure rates and inherent revision arthroplasties. Novel bioelectronic devices that include biophysical stimulators (such as electric stimulators) and sensing systems are desired since these will allow for long-term monitoring and control of the bone-implant interface, in a personalized manner. We have developed acting-sensing dual systems operated at high frequency (HF) that are able to stimulate osteoconduction in pre-osteoblasts and osteoinduction in human adipose-derived mesenchymal stem cells (hASCs). The project included – to our knowledge – the first-time proteomic analysis of microvesicles secreted from osteoblasts electrically-stimulated in vitro by a capacitive stimulator of thin interdigitated electrodes delivering an electrical 60 kHz HF stimulation, 30 min/day. Results revealed regulation of osteodifferentiation and mineralization-related proteins (e.g. Tgfb3, Ttyh3, Itih1, Aldh1a1).
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:52:40.320.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD028551
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterBárbara M de Sousa
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListiodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-09-16 07:39:07ID requested
12021-11-29 06:20:07announced
22021-12-03 01:30:45announced2021-12-03: Updated publication reference for DOI(s): 10.1038/s41536-021-00184-6.
32021-12-15 07:30:25announced2021-12-03: Updated publication reference for DOI(s): 10.1038/s41536-021-00184-6.
2021-12-15: Updated publication reference for PubMed record(s): 34815414.
2021-12-15: Updated publication reference for DOI(s): 10.1038/s41536-021-00184-6.
42023-11-14 08:52:41announced2023-11-14: Updated project metadata.
Publication List
10.6019/PXD028551;
de Sousa BM, Correia CR, Ferreira JAF, Mano JF, Furlani EP, Soares Dos Santos MP, Vieira SI, Capacitive interdigitated system of high osteoinductive/conductive performance for personalized acting-sensing implants. NPJ Regen Med, 6(1):80(2021) [pubmed]
Keyword List
submitter keyword: extracellular, capacitive coupling, electrical stimulation, bone, osteoblasts, matrix microvesicles, LC-MS/MS
Contact List
Sandra I. Vieira
contact affiliationDepartment of Medical Sciences, Institute of Biomedicine (iBiMED), University of Aveiro, 3810-193 Aveiro, Portugal.
contact emailsivieira@ua.pt
lab head
Bárbara M de Sousa
contact affiliationInstitute of Biomedicine, Department of Medical Sciences, University of Aveiro, Portugal
contact emailbmsousa@ua.pt
dataset submitter
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