PXD028545

PXD028545 is an original dataset announced via ProteomeXchange.

Title	Comparative proteomic analysis of plasma membrane proteins in rice leaves reveals a vesicle trafficking network in plant immunity provoked by blast fungi
Description	This project aims to analyze rice plasma membrane proteins related to resistance against rice blast infection. We extracted rice plasma membrane proteins before and after M.oryzae infection for 24h, and then used trypsin to digest and iTRAQ to label the peptides, HPLC-MS/MS was used to seperate and identify peptides. 1.1/0.909 fold change with p-value < 0.05 was used as threshold for differentially expressed proteins. 2,977 proteins were identified and 951 of which were found to be responsive to resistance against M. oryzae. Gene Ontology (GO) enrichment analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis and protein interaction network showed that plenty of proteins were involved in vesicle trafficking with obvious functional tendencies towards transport, vesicle-mediated transport, secretion, endocytosis and phagosome. 10 DEPs were validated at transcript level, and a SNARE protein named NPSN (novel plant-specific SNARE)13 actively responded to M. oryzae infection, and it contributed to rice blast resistance and mainly located at PM.
HostingRepository	PRIDE
AnnounceDate	2022-05-19
AnnouncementXML	Submission_2022-05-19_11:06:43.670.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhi Zhao
SpeciesList	scientific name: Oryza sativa (Rice); NCBI TaxID: 4530;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-16 06:44:05	ID requested
⏵ 1	2022-05-19 11:06:44	announced

Zhao Z, Li M, Zhang H, Yu Y, Ma L, Wang W, Fan Y, Huang N, Wang X, Liu K, Dong S, Tang H, Wang J, Zhang H, Bao Y, Comparative Proteomic Analysis of Plasma Membrane Proteins in Rice Leaves Reveals a Vesicle Trafficking Network in Plant Immunity That Is Provoked by Blast Fungi. Front Plant Sci, 13():853195(2022) [pubmed]

submitter keyword: rice, plasma membrane, LC-MS/MS

Yongmei Bao
contact affiliation	State key laboratory of crop genetics and germplasm enhancement, college of agriculture, Nanjing Agricultural University, China
contact email	yongmeibao@njau.edu.cn
lab head
Zhi Zhao
contact affiliation	Nanjing Agricultural University
contact email	2020101064@stu.njau.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/05/PXD028545

PRIDE project URI

• PRIDE
  1. PXD028545
     1. Label: PRIDE project
     2. Name: Comparative proteomic analysis of plasma membrane proteins in rice leaves reveals a vesicle trafficking network in plant immunity provoked by blast fungi