PXD028534

PXD028534 is an original dataset announced via ProteomeXchange.

Title	Phosphorylation of the FACT histone chaperone subunit SPT16 modulates Arabidopsis development and affects chromatin at RNA polymerase II transcriptional start sites
Description	The heterodimeric histone chaperone FACT consisting of SSRP1 and SPT16 contributes to dynamic nucleosome rearrangements during various DNA-dependent processes including transcription. In search of post-translational modifications that may regulate the activity of FACT, SSRP1 and SPT16 were isolated from Arabidopsis cells and analysed by mass spectrometry. Four acetylated lysine residues could be mapped within the basic C-terminal region of SSRP1, while three phosphorylated serine/threonine residues were identified in the acidic C-terminal region of SPT16. Mutational analysis of the SSRP1 acetylation sites revealed only mild effects. However, phosphorylation of SPT16 that is catalysed by protein kinase CK2, modulates histone interactions. A non-phosphorylatable version of SPT16 displayed reduced histone binding and (unlike the phosphorylatable wild-type and phosphomimic versions) proved inactive in complementing the growth and developmental phenotypes of spt16 mutant plants. In plants expressing the non-phosphorylatable SPT16 version we detected at a subset of genes enrichment of histone H3 directly upstream of RNA polymerase II transcriptional start sites (TSSs) in a region that usually is nucleosome-depleted. This is associated with altered transcript levels, suggesting that some genes require phosphorylation of the SPT16 acidic region for establishing the correct nucleosome occupancy at the TSS as a prerequisite for proper transcription.
HostingRepository	PRIDE
AnnounceDate	2024-10-07
AnnouncementXML	Submission_2024-10-07_13:42:35.020.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD028534
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Andrey Tvardovskiy
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	monomethylated residue; phosphorylated residue; acetylated residue; monohydroxylated residue; dimethylated residue; N6,N6,N6-trimethyl-L-lysine; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-16 06:42:17	ID requested
⏵ 1	2024-10-07 13:42:35	announced

Michl-Holzinger P, Obermeyer S, Markusch H, Pfab A, Ettner A, Bruckmann A, Babl S, L, ä, ngst G, Schwartz U, Tvardovskiy A, Jensen ON, Osakabe A, Berger F, Grasser KD, Phosphorylation of the FACT histone chaperone subunit SPT16 affects chromatin at RNA polymerase II transcriptional start sites in Arabidopsis. Nucleic Acids Res, 50(9):5014-5028(2022) [pubmed]

10.1093/nar/gkac293;

10.6019/PXD028534;

submitter keyword: Arabidopsis,FACT complex, acetylation, phosphorylation

Klaus Grasser
contact affiliation	Department of Cell Biology & Plant Biochemistry, Center for Biochemistry, University of Regensburg, Universitätsstr. 31, D-93053 Regensburg, Germany
contact email	Klaus.Grasser@biologie.uni-regensburg.de
lab head
Andrey Tvardovskiy
contact affiliation	Institute of Functional Epigenetics, Helmholtz Zentrum München, Neuherberg 85764, Germany
contact email	andrey.tvardovskiy@helmholtz-muenchen.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD028534
     1. Label: PRIDE project
     2. Name: Phosphorylation of the FACT histone chaperone subunit SPT16 modulates Arabidopsis development and affects chromatin at RNA polymerase II transcriptional start sites