PXD028534 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Phosphorylation of the FACT histone chaperone subunit SPT16 modulates Arabidopsis development and affects chromatin at RNA polymerase II transcriptional start sites |
Description | The heterodimeric histone chaperone FACT consisting of SSRP1 and SPT16 contributes to dynamic nucleosome rearrangements during various DNA-dependent processes including transcription. In search of post-translational modifications that may regulate the activity of FACT, SSRP1 and SPT16 were isolated from Arabidopsis cells and analysed by mass spectrometry. Four acetylated lysine residues could be mapped within the basic C-terminal region of SSRP1, while three phosphorylated serine/threonine residues were identified in the acidic C-terminal region of SPT16. Mutational analysis of the SSRP1 acetylation sites revealed only mild effects. However, phosphorylation of SPT16 that is catalysed by protein kinase CK2, modulates histone interactions. A non-phosphorylatable version of SPT16 displayed reduced histone binding and (unlike the phosphorylatable wild-type and phosphomimic versions) proved inactive in complementing the growth and developmental phenotypes of spt16 mutant plants. In plants expressing the non-phosphorylatable SPT16 version we detected at a subset of genes enrichment of histone H3 directly upstream of RNA polymerase II transcriptional start sites (TSSs) in a region that usually is nucleosome-depleted. This is associated with altered transcript levels, suggesting that some genes require phosphorylation of the SPT16 acidic region for establishing the correct nucleosome occupancy at the TSS as a prerequisite for proper transcription. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-07 |
AnnouncementXML | Submission_2024-10-07_13:42:35.020.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD028534 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Andrey Tvardovskiy |
SpeciesList | scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; |
ModificationList | monomethylated residue; phosphorylated residue; acetylated residue; monohydroxylated residue; dimethylated residue; N6,N6,N6-trimethyl-L-lysine; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-09-16 06:42:17 | ID requested | |
⏵ 1 | 2024-10-07 13:42:35 | announced | |
Publication List
Michl-Holzinger P, Obermeyer S, Markusch H, Pfab A, Ettner A, Bruckmann A, Babl S, L, ä, ngst G, Schwartz U, Tvardovskiy A, Jensen ON, Osakabe A, Berger F, Grasser KD, Phosphorylation of the FACT histone chaperone subunit SPT16 affects chromatin at RNA polymerase II transcriptional start sites in Arabidopsis. Nucleic Acids Res, 50(9):5014-5028(2022) [pubmed] |
10.1093/nar/gkac293; |
10.6019/PXD028534; |
Keyword List
submitter keyword: Arabidopsis,FACT complex, acetylation, phosphorylation |
Contact List
Klaus Grasser |
contact affiliation | Department of Cell Biology & Plant Biochemistry, Center for Biochemistry, University of Regensburg, Universitätsstr. 31, D-93053 Regensburg, Germany |
contact email | Klaus.Grasser@biologie.uni-regensburg.de |
lab head | |
Andrey Tvardovskiy |
contact affiliation | Institute of Functional Epigenetics, Helmholtz Zentrum München, Neuherberg 85764, Germany |
contact email | andrey.tvardovskiy@helmholtz-muenchen.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD028534
- Label: PRIDE project
- Name: Phosphorylation of the FACT histone chaperone subunit SPT16 modulates Arabidopsis development and affects chromatin at RNA polymerase II transcriptional start sites