PXD028522

PXD028522 is an original dataset announced via ProteomeXchange.

Title	Molecular architecture of the antiophidic protein DM64 and its binding specificity to myotoxin II from Bothrops asper venom
Description	DM64 is a toxin-neutralizing serum glycoprotein isolated from the South American opossum Didelphis aurita. This ophiophagous marsupial is naturally resistant to snake envenomation and has evolved such biochemical defense as a trophic adaptation. The endogenous antitoxin is a human α1B-glycoprotein homolog of 64 kDa, specifically targeting myotoxic phospholipases A2, which account for most local tissue damage associated with viper snakebites. This study investigated the noncovalent complex formed between native DM64 and myotoxin II, a myotoxic calcium-independent phospholipase-like protein from Bothrops asper venom. Analytical ultracentrifugation, size exclusion chromatography, and small-angle X-ray scattering (SAXS) data indicated that DM64 is monomeric in solution and binds equimolar amounts of the toxin. Initial attempts to solve the structure of DM64 experimentally failed due to technical limitations caused by N-glycan heterogeneity and low recovery of heterologous protein. Classical molecular modeling techniques were impaired by the lack of templates with more than 25% sequence identity with DM64. An integrative structural biology approach was then applied to generate a refined three-dimensional model of the inhibitor bound to myotoxin II. The five immunoglobulin-like domains of DM64 were individually modeled using the I-TASSER suite. Distance constraints generated by cross-linking mass spectrometry of the complex guided the docking of DM64 domains to the crystal structure of myotoxin II (1CLP), using the Rosetta docking suite. The model of the DM64-myotoxin II complex was successfully fitted to a SAXS envelope. Inter-protein cross-links and limited hydrolysis analyses shed light on the inhibitor's regions involved with toxin interaction, revealing the critical participation of the first, third, and fifth domains of DM64. Our data showed that the fifth domain of DM64 binds to myotoxin II’s amino-terminal and -wing regions. The third domain of the inhibitor acts in a complementary way to the fifth domain. Their binding to these toxin regions presumably interferes with dimerization, a critical step in the allosteric activation of myotoxic PLA2-like proteins. Additionally, the first domain of DM64 likely interacts with the functional site of the toxin putatively associated with membrane anchorage. We propose that both mechanisms concur to effectively inhibit myotoxin II toxicity by DM64 binding. In summary, we argue such topological characterization of this toxin-antitoxin complex to constitute an essential first step toward the rational design of novel peptide-based antivenom therapies targeting snake venom myotoxins.
HostingRepository	PRIDE
AnnounceDate	2022-01-20
AnnouncementXML	Submission_2022-01-20_09:38:51.889.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Viviane Bastos
SpeciesList	scientific name: Didelphis aurita; NCBI TaxID: 85694; scientific name: Bothrops asper; NCBI TaxID: 8722;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-16 05:15:17	ID requested
⏵ 1	2022-01-20 09:38:52	announced

Dataset with its publication pending

submitter keyword: Snake venom, toxin, inhibitor, protein cross-linking, mass-spectrometry, molecular modeling, structure-function, structural model

Ana Gisele da Costa Neves Ferreira
contact affiliation	Head of the Laboratory of Toxinology Oswaldo Cruz Institute Oswaldo Cruz Foundation (FIOCRUZ) Rio de Janeiro, Brazil.
contact email	anag@ioc.fiocruz.br
lab head
Viviane Bastos
contact affiliation	Oswaldo Cruz Foundation
contact email	vivika.bastos@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD028522
     1. Label: PRIDE project
     2. Name: Molecular architecture of the antiophidic protein DM64 and its binding specificity to myotoxin II from Bothrops asper venom