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PXD028519

PXD028519 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDegradations of different processed Mg alloy implants lead to distinct foreign body reactions (FBRs)
DescriptionMg alloy is one of the lightest metals on earth and has the most similar mechanical properties to human bones. However, the active chemical properties and fast in vivo degrade speed significantly hinder its further employment as human implant. In this study, three different Mg alloys are employed to investigate the in vitro corrosion behavior including weight loss, pH value evolution and surface hardness and in vivo degradable speed and tissue compatibility. All three Mg alloys are well tolerated when implanted in SD rats, with minimal influence on hepato-and renal functions and vital organs. Compared to cold extruded AZ31 (CE AZ31) and pure Mg (PM) specimens, fully annealed AZ31 (FA AZ31) presents much stable surface asperity. Proteomics analysis of tissues near implant site showed that FA AZ31 activates few inflammation and immune associated signaling pathways; while the CE AZ31 and pure Mg led more significant inflammatory responses as confirmed by the cytokine array that pure Mg stimulate higher IL-1 production than FA AZ31. Further, FA AZ31 can activate pathways of cell organization and development that may improve the recovery of the injured tissues neighboring the implant sites. Besides, all three Mg alloys cannot inhibit a methicillin-resistant S. aureus growth if the implants are contaminated with this bacterium. FA AZ31 has a higher ratio of first-order pyramidal slips system (10-11) {10-1-2} than pure Mg and cold extruded AZ31 analysed by EBSD, suggesting the process of dynamic recovery may plays an important role in the improvement of mechanical properties, especially bio-properties including corrosion resistance and biocompatibility. In conclusion, FA AZ31 has better biocompatibilities and corrosion resistance, makes it a promising candidate of metal-based degradable implant and warrant further investigation.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_07:36:33.267.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterTianfang Wang
SpeciesList scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue; acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-09-16 03:01:21ID requested
12023-01-26 14:18:18announced
22023-11-14 07:36:33announced2023-11-14: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: rat, TMT10plex, foreign body reactions, Mg alloy, LC-MS/MS
Contact List
Tianfang Wang
contact affiliationGenecology Research Centre, University of the Sunshine Coast
contact emailtwang@usc.edu.au
lab head
Tianfang Wang
contact affiliationUniversity of the Sunshine Coast
contact emailtwang@usc.edu.au
dataset submitter
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Dataset FTP location
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