PXD028518

PXD028518 is an original dataset announced via ProteomeXchange.

Title	Eccrine sweat proteomics for the diagnosis of cystic fibrosis
Description	In clinical routine, the diagnosis of cystic fibrosis (CF) is still a challenge due to the limitations of diagnosis guidelines and tests. A diagnosis test of choice, the sweat test measures eccrine sweat chloride concentration as a byproduct of the eccrine sweat gland CFTR function. Despite the combined use of CFTR genotyping and direct physiologic testing of CFTR function, reports of inconclusive diagnosis justified the need for alternative tests and new biomarkers. Meanwhile, eccrine sweat composition has already been linked to disease-specific profiles of non-electrolytes (i.e. proteins, peptides and metabolites). In this study, we analyzed sweat samples from 28 healthy volunteers and 14 CF patients by UHPLC-Q-Orbitrap-based Shotgun proteomics, to address CF-related changes in sweat protein composition and abundance. Over 1000 proteins were identified and quantified in a label-free manner. Beside similar protein composition, enrichment and functional classifications, HV and CF samples were grouped apart since protein abundance profiles were significantly correlated with CF status and degree of severity (ΔF508 homozygous and pancreatic insufficiency onset). Four-hundred and two proteins in CF-specific abundance, 68 proteins in genotype-specific abundance and 71 proteins in abundance related to pancreatic status, respectively, highlighted eccrine gland cell perturbations in protein biosynthesis & trafficking, CFTR proteostasis & membrane stability, cell-cell adherence, membrane integrity & cytoskeleton crosstalk. Cytoskeleton-related biomarkers were of utmost interest because of consistent abundances between CF sweat and other CF tissues. Nine clinical CF diagnosis biomarker (CUTA, ARG1, EZR, AGA, FLNA, MAN1A1, MIA3, LFNG, SIAE) and 5 CF severity biomarker (ARG1, GPT, MDH2, EML4 (ΔF508 homozygous), MGAT1 (pancreatic insufficiency)) candidates were deemed suitable for further verification.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:05:28.638.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD028518
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Bastien Burat
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-16 03:00:46	ID requested
1	2022-10-13 13:59:51	announced
⏵ 2	2023-11-14 07:05:30	announced	2023-11-14: Updated project metadata.

Burat B, Reynaerts A, Baiwir D, Fl, é, ron M, Gohy S, Eppe G, Leal T, Mazzucchelli G, Sweat Proteomics in Cystic Fibrosis: Discovering Companion Biomarkers for Precision Medicine and Therapeutic Development. Cells, 11(15):(2022) [pubmed]

10.6019/PXD028518;

submitter keyword: Human, Orbitrap, Biomarkers,Eccrine sweat, Cystic Fibrosis, Shotgun proteomics

Gauthier EPPE
contact affiliation	Laboratory of Mass Spectrometry-Director MolSys Research Unit-Director CART (Center for Analytical Research and Technology)-Director Liège Université, Liège, Belgium
contact email	g.eppe@uliege.be
lab head
Bastien Burat
contact affiliation	MSLab, MolSys RU, University of Liege
contact email	bastien.burat@etu.unilim.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD028518

PRIDE project URI

• PRIDE
  1. PXD028518
     1. Label: PRIDE project
     2. Name: Eccrine sweat proteomics for the diagnosis of cystic fibrosis