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PXD028509

PXD028509 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleH. pylori utilizes membrane vesicles as a novel tool to extrude bismuth drug
DescriptionAntibiotic resistance is one of the major threats to human health. Combination of antibiotics with metals is among the proposed alternative treatments. However, so far only one such combination with the metal bismuth successfully used in clinics against Helicobacter pylori. This bacterial pathogen colonizes the human stomach potentially resulting in gastric cancer that causes 800,000 deaths every year. The effect of bismuth on H. pylori is not understood, for sub-lethal doses, such as those present in the plasma of treated patients. We addressed this question and observed that bismuth induces the formation of homogeneous membrane vesicles (MVs) with unique protein cargo content enriched in bismuth-binding proteins. To our surprise, MVs concentrate bismuth, unlike bacterial cells from which they originate. Our results revealed a novel function of MVs in metal detoxification where bismuth is accumulated to discard it from bacteria. Polyphosphate granules (poly-P) formed upon bismuth exposure are associated with changes in nucleoid structure and cell segregation. Thus, even low doses of bismuth induce profound changes in H. pylori physiology and highlight novel defense mechanisms employed by bacteria against bismuth toxicity.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:52:39.320.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMagalie Duchateau
SpeciesList scientific name: Helicobacter pylori G27; NCBI TaxID: 563041;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-09-16 02:32:05ID requested
12022-09-19 02:29:15announced
22023-11-14 08:52:40announced2023-11-14: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: bismuth,Helicobater pylori, antibiotic resistance, membrane vesicle
Contact List
Hilde DE REUSE
contact affiliationUnité "Pathogenèse de Helicobacter" Department of Microbiology CNRS UMR2001 Institut Pasteur 28 rue du Dr. Roux 75724 Paris cedex 15
contact emailhilde.de-reuse@pasteur.fr
lab head
Magalie Duchateau
contact affiliationInstitut Pasteur
contact emailmagalie.duchateau@pasteur.fr
dataset submitter
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Dataset FTP location
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