PXD028506

PXD028506 is an original dataset announced via ProteomeXchange.

Title	Evaluation of Bacillus velezensis Kos for the control of Lecanicillium fungicola
Description	Lecanicillium fungicola, the causative agent of dry bubble disease on Agaricus bisporus results in significant crop losses for mushroom growers worldwide. Dry bubble disease is treated through strict hygiene control methods and the application of chemical fungicides but an increase in fungicide resistant L. fungicola strains has resulted in a need to develop alternative biocontrol treatment methods. The aim of the work presented here was to evaluate the response of L. fungicola to a Bacillus velezensis isolate to assess its potential as a novel biocontrol agent. The bacterial species in Serenade, a commercially available biocontrol treatment was also included in this analysis. Exposure of 48 hr L. fungicola cultures to 25% v/v 96h B. velezensis culture filtrate resulted in a 45% reduction in biomass (P < 0.0002) and a disruption in hyphal structure and morphology. Characterisation of the proteomic response of L. fungicola following exposure to B. velezensis culture filtrate revealed an increase in the abundance of a variety of proteins associated with stress response (Norsolorinic acid reductase (+8 fold), isocitrate lyase (+7 fold) and MMS19 nucleotide excision repair protein (+4 fold). There was also a decrease in the abundance of proteins associated with transcription (40S ribosomal protein S30 (-33 fold), 60S ribosomal protein L5 (-45 foldThe results presented here indicate that B. velezensis culture filtrate is capable of inhibiting the growth of L. fungicola and inducing a stress response, thus indicating its potential to control this important pathogen of mushrooms.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:37:20.265.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joy Clarke
SpeciesList	scientific name: Lecanicillium fungicola var. fungicola; NCBI TaxID: 170721;
ModificationList	monohydroxylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-16 02:27:37	ID requested
1	2022-06-01 02:16:48	announced
⏵ 2	2024-10-22 05:37:20	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Agaricus bisporus, Lecanicillium fungicola, proteomics, Bacillus velezensis

Kevin Kavanagh
contact affiliation	Lab PI
contact email	kevin.kavanagh@mu.ie
lab head
Joy Clarke
contact affiliation	Maynooth University
contact email	joy.clarke.2017@mumail.ie
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/06/PXD028506

PRIDE project URI

• PRIDE
  1. PXD028506
     1. Label: PRIDE project
     2. Name: Evaluation of Bacillus velezensis Kos for the control of Lecanicillium fungicola