PXD028498

PXD028498 is an original dataset announced via ProteomeXchange.

Title	Identification of proline to serine mistranslation in Drosophila melanogaster pupae
Description	Transfer RNAs (tRNAs) are the adaptor molecules required for reading of the genetic code and the accurate production of proteins. tRNA variants can lead to genome-wide mistranslation, the misincorporation of amino acids not specified by the standard genetic code, into nascent proteins. While genome sequencing has identified putative mistranslating tRNA variants in human populations, little is known regarding how mistranslation affects multicellular organisms. Here, we create a Drosophila melanogaster model for mistranslation by integrating a serine tRNA variant that mistranslates serine for proline (tRNA(Ser)[UGG, G26A]) into the fly genome. Using mass spectrometry, we find that tRNA(Ser)[UGG, G26A] misincorporates serine for proline at a frequency of ~ 0.6% per codon. This model will enable studies into the synergistic effects of mistranslating tRNA variants and disease-causing alleles.
HostingRepository	PRIDE
AnnounceDate	2021-09-18
AnnouncementXML	Submission_2021-09-18_12:23:40.044.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Matthew Berg
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	L-methionine sulfone; S-carboxamidomethyl-L-cysteine
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-16 00:21:00	ID requested
⏵ 1	2021-09-18 12:23:40	announced

Dataset with its publication pending

submitter keyword: Drosophila melanogaster, pupae, mistranslation, tRNA variants

Judit Villen
contact affiliation	Department of Genome Science, University of Washington, Seattle, USA
contact email	jvillen@uw.edu
lab head
Matthew Berg
contact affiliation	Department of Genome Sciences, University of Washington
contact email	mattberg@uw.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD028498
     1. Label: PRIDE project
     2. Name: Identification of proline to serine mistranslation in Drosophila melanogaster pupae