PXD028492

PXD028492 is an original dataset announced via ProteomeXchange.

Title	Cul5 regulates CD4+ T cell fate choice and allergic inflammation
Description	Antigen encounter directs CD4+ T cells to differentiate into T-helper or -regulatory cells. This process focuses the immune response on the invading pathogen and limits tissue damage. Mechanisms that govern -helper versus -regulatory fate remain poorly understood. Here, we show that the E3 ubiquitin ligase Cul5 determines fate selection in CD4+ T cells by regulating IL-4 receptor signaling. Mice lacking Cul5 in T cells developed enhanced Th2 and Th9 inflammation and pathophysiological features of atopic asthma upon allergen exposure. Following T cell activation, Cul5 formed a complex with CIS and pJak1. Loss of Cul5 function resulted in reduced ubiquitylation and increased stability of pJak1, elevated STAT6 activity, and a reduced threshold for IL-4 receptor signaling. In keeping with this, Cul5-deficient T cells deviated from Treg to Th9 differentiation in low IL-4 conditions. These data support that Cul5 promotes a tolerogenic T cell fate choice and reduces susceptibility to allergic asthma.
HostingRepository	PRIDE
AnnounceDate	2022-06-09
AnnouncementXML	Submission_2022-06-09_02:53:56.043.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hossein Fazelinia
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-15 23:24:33	ID requested
⏵ 1	2022-06-09 02:53:57	announced

Kumar B, Field NS, Kim DD, Dar AA, Chen Y, Suresh A, Pastore CF, Hung LY, Porter N, Sawada K, Shah P, Elbulok O, Moser EK, Herbert DR, Oliver PM, T cell fate choice and allergic inflammation. Nat Commun, 13(1):2786(2022) [pubmed]

submitter keyword: T-cell, CD4, Ubiquitin, Cul5, CRL5, CIS, Asthma, Allergy

Paula M. Oliver, PhD
contact affiliation	Cell Pathology Division 816F/ARC Children’s Hospital of Philadelphia The University of Pennsylvania 3615 Civic Center Blvd. 816F/ARC Philadelphia, PA 19104 Office: 267-426-2839 Fax: 267-426-5165
contact email	paulao@mail.med.upenn.edu
lab head
Hossein Fazelinia
contact affiliation	Children's Hospital of Philadelphia
contact email	fazeliniah@email.chop.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/06/PXD028492

PRIDE project URI

• PRIDE
  1. PXD028492
     1. Label: PRIDE project
     2. Name: Cul5 regulates CD4+ T cell fate choice and allergic inflammation