PXD028492 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Cul5 regulates CD4+ T cell fate choice and allergic inflammation |
Description | Antigen encounter directs CD4+ T cells to differentiate into T-helper or -regulatory cells. This process focuses the immune response on the invading pathogen and limits tissue damage. Mechanisms that govern -helper versus -regulatory fate remain poorly understood. Here, we show that the E3 ubiquitin ligase Cul5 determines fate selection in CD4+ T cells by regulating IL-4 receptor signaling. Mice lacking Cul5 in T cells developed enhanced Th2 and Th9 inflammation and pathophysiological features of atopic asthma upon allergen exposure. Following T cell activation, Cul5 formed a complex with CIS and pJak1. Loss of Cul5 function resulted in reduced ubiquitylation and increased stability of pJak1, elevated STAT6 activity, and a reduced threshold for IL-4 receptor signaling. In keeping with this, Cul5-deficient T cells deviated from Treg to Th9 differentiation in low IL-4 conditions. These data support that Cul5 promotes a tolerogenic T cell fate choice and reduces susceptibility to allergic asthma. |
HostingRepository | PRIDE |
AnnounceDate | 2022-06-09 |
AnnouncementXML | Submission_2022-06-09_02:53:56.043.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Hossein Fazelinia |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-09-15 23:24:33 | ID requested | |
⏵ 1 | 2022-06-09 02:53:57 | announced | |
Publication List
Kumar B, Field NS, Kim DD, Dar AA, Chen Y, Suresh A, Pastore CF, Hung LY, Porter N, Sawada K, Shah P, Elbulok O, Moser EK, Herbert DR, Oliver PM, T cell fate choice and allergic inflammation. Nat Commun, 13(1):2786(2022) [pubmed] |
Keyword List
submitter keyword: T-cell, CD4, Ubiquitin, Cul5, CRL5, CIS, Asthma, Allergy |
Contact List
Paula M. Oliver, PhD |
contact affiliation | Cell Pathology Division 816F/ARC Children’s Hospital of Philadelphia The University of Pennsylvania 3615 Civic Center Blvd. 816F/ARC Philadelphia, PA 19104 Office: 267-426-2839 Fax: 267-426-5165 |
contact email | paulao@mail.med.upenn.edu |
lab head | |
Hossein Fazelinia |
contact affiliation | Children's Hospital of Philadelphia |
contact email | fazeliniah@email.chop.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD028492
- Label: PRIDE project
- Name: Cul5 regulates CD4+ T cell fate choice and allergic inflammation