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PXD028492

PXD028492 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCul5 regulates CD4+ T cell fate choice and allergic inflammation
DescriptionAntigen encounter directs CD4+ T cells to differentiate into T-helper or -regulatory cells. This process focuses the immune response on the invading pathogen and limits tissue damage. Mechanisms that govern -helper versus -regulatory fate remain poorly understood. Here, we show that the E3 ubiquitin ligase Cul5 determines fate selection in CD4+ T cells by regulating IL-4 receptor signaling. Mice lacking Cul5 in T cells developed enhanced Th2 and Th9 inflammation and pathophysiological features of atopic asthma upon allergen exposure. Following T cell activation, Cul5 formed a complex with CIS and pJak1. Loss of Cul5 function resulted in reduced ubiquitylation and increased stability of pJak1, elevated STAT6 activity, and a reduced threshold for IL-4 receptor signaling. In keeping with this, Cul5-deficient T cells deviated from Treg to Th9 differentiation in low IL-4 conditions. These data support that Cul5 promotes a tolerogenic T cell fate choice and reduces susceptibility to allergic asthma.
HostingRepositoryPRIDE
AnnounceDate2022-06-09
AnnouncementXMLSubmission_2022-06-09_02:53:56.043.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterHossein Fazelinia
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-09-15 23:24:33ID requested
12022-06-09 02:53:57announced
Publication List
Kumar B, Field NS, Kim DD, Dar AA, Chen Y, Suresh A, Pastore CF, Hung LY, Porter N, Sawada K, Shah P, Elbulok O, Moser EK, Herbert DR, Oliver PM, T cell fate choice and allergic inflammation. Nat Commun, 13(1):2786(2022) [pubmed]
Keyword List
submitter keyword: T-cell, CD4, Ubiquitin, Cul5, CRL5, CIS, Asthma, Allergy
Contact List
Paula M. Oliver, PhD
contact affiliationCell Pathology Division 816F/ARC Children’s Hospital of Philadelphia The University of Pennsylvania 3615 Civic Center Blvd. 816F/ARC Philadelphia, PA 19104 Office: 267-426-2839 Fax: 267-426-5165
contact emailpaulao@mail.med.upenn.edu
lab head
Hossein Fazelinia
contact affiliationChildren's Hospital of Philadelphia
contact emailfazeliniah@email.chop.edu
dataset submitter
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Dataset FTP location
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Repository Record List
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