PXD028490

PXD028490 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteome profiling using the isobaric carrier without the need for phosphoenrichment
Description	Phosphorylation is a crucial component of signaling cascades in a cell. It controls various biological cellular functions such as cell growth and apoptosis. Due to the low stoichiometry of phosphorylaion, enrichment of phosphopeptide prior to LC-MS/MS is necessary for comprehensive phosphoproteome analysis and typical quantitative phosphoproteomic workflows are often limited by the amount of sample input. To address this issue, we developed an easy-to-establish, widely applicable and reproducible strategy to amplify phosphoproteome signals from a small amount of sample without phosphoenrichment step. Taking advantage of the multiplexing nature of isobaric labeling to achieve merged signal from multiple samples, and using a large amount of enriched phosphopeptides as a carrier, we were able to amplify a trace amount of phosphopeptide in the unpurified sample to an identifiable level and quantify it with the reporter ion intensity of the isobaric tag. Our result showed that >1400 phosphopeptide were quantified from 250 ng of tryptic peptides of cell extract. Through proof-of-concept experiments of our strategy, we distinguished 3 types of lung cancer cell line based on their quantitative phosphoproteome data and also identified phosphoproteome changes upon cellular perturbation achieved by drug treatment.
HostingRepository	PRIDE
AnnounceDate	2022-03-07
AnnouncementXML	Submission_2022-03-07_12:53:52.334.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yumi Kwon
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-15 23:15:46	ID requested
⏵ 1	2022-03-07 12:53:53	announced

Kwon Y, Lee S, Park N, Ju S, Shin S, Yoo S, Lee H, Lee C, Phosphoproteome Profiling Using an Isobaric Carrier without the Need for Phosphoenrichment. Anal Chem, 94(10):4192-4200(2022) [pubmed]

submitter keyword: Phosphoproteome, isobaric labeling, TMT, carrier, quantitative proteomics

Cheolju Lee
contact affiliation	Center for theragnosis, Korea Institute of Science and Technology
contact email	clee270@kist.re.kr
lab head
Yumi Kwon
contact affiliation	Korea Institute of Science and Technology
contact email	ymkwon@kist.re.kr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD028490
     1. Label: PRIDE project
     2. Name: Phosphoproteome profiling using the isobaric carrier without the need for phosphoenrichment