PXD028489

PXD028489 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Post-transcriptionally regulated genes are essential for pluripotent stem cell survival
Description	Here, by combining transcriptome and proteome profiling, we identified 228 post-transcriptionally regulated genes with strict regulation at the protein level in PSCs. Among them, 84 genes were found necessary for the survival of several cell types including PSCs, and 20 genes were identified as specific for the survival of PSCs. These 20 proteins were upregulated only in PSCs and not in differentiated cells derived from the three germ layers. Subcellular fractionation of the mRNA showed that the expression level of most of the 20 proteins was regulated at the mRNA localization stage from the nucleus to the cytoplasm in PSCs, but their translation efficiency was unchanged compared with other cell types. Together, these results reveal a critical function of post-transcriptionally regulated genes on PSC survival.
HostingRepository	jPOST
AnnounceDate	2022-05-10
AnnouncementXML	Submission_2022-11-20_17:59:18.920.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Iwasaki Mio
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; deamidated L-asparagine; deamidated L-glutamine; 2-pyrrolidone-5-carboxylic acid (Glu); 2-pyrrolidone-5-carboxylic acid (Gln); alpha-amino acetylated residue; Applied Biosystems iTRAQ(TM) multiplexed quantitation chemistry
Instrument	TripleTOF 5600

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-15 22:56:01	ID requested
1	2022-05-09 08:00:05	announced
2	2022-09-18 02:49:44	announced	2022-09-18: Updated FTP location.
⏵ 3	2022-11-20 17:59:19	announced	2022-11-21: Updated PubMed.

Publication List

Iwasaki M, Kawahara Y, Okubo C, Yamakawa T, Nakamura M, Tabata T, Nishi Y, Narita M, Ohta A, Saito H, Yamamoto T, Nakagawa M, Yamanaka S, Takahashi K, Multi-omics approach reveals posttranscriptionally regulated genes are essential for human pluripotent stem cells. iScience, 25(5):104289(2022) [pubmed]

Iwasaki M, Kawahara Y, Okubo C, Yamakawa T, Nakamura M, Tabata T, Nishi Y, Narita M, Ohta A, Saito H, Yamamoto T, Nakagawa M, Yamanaka S, Takahashi K, Multi-omics approach reveals posttranscriptionally regulated genes are essential for human pluripotent stem cells. iScience, 25(5):104289(2022) [pubmed]

Keyword List

submitter keyword: iPSC, iTRAQ, post-transcriptional regulation

submitter keyword: iPSC, iTRAQ, post-transcriptional regulation

Contact List

Mio Iwasaki
lab head
Iwasaki Mio
contact affiliation	Kyoto University
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001308/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001308/