PXD028465 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Streptococcus pneumoniae and IAV co infection induces altered polyubiquitination in A549 cells |
Description | Epithelial cells are the first line of defense within the lung. Disruption of the epithelial barrier by pathogens enables the systematic dissemination of bacteria or viruses within the host, leading to severe diseases with fatal outcomes. Thus, the lung epithelium can be damaged by seasonal and pandemic influenza A viruses. Influenza A virus infection induced dysregulation of the immune system is beneficial for the dissemination of bacteria to the lower respiratory tract, causing bacterial and viral co-infection. Host cells regulate protein homeostasis and the response to different stimuli, for instance pathogen infections, by post translational modification of proteins. Aside from protein phosphorylation, ubiquitination of proteins is an essential regulatory tool in virtually every cellular process, such as protein homeostasis, the host immune response, cell morphology, and in clearing of cytosolic pathogens. Here, we analyzed the proteome and ubiquitinome of A549 cells in response to Streptococcus pneumoniae D39 Δcps and influenza A virus H1N1 as well as bacterial and viral co-infection. Pneumococcal infection induced alterations in the ubiquitination of proteins involved in the organization of the actin cytoskeleton and Rho GTPases, but had minor effects on the abundance of host proteins. H1N1 infection is reflected by an anti-viral state of A549 cells. Finally, co infection resembled the imprints of both infecting pathogens with a minor increase in the observed alterations in protein and ubiquitination abundance. |
HostingRepository | PRIDE |
AnnounceDate | 2022-02-24 |
AnnouncementXML | Submission_2022-02-24_14:26:41.504.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Thomas Sura |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-09-14 15:37:04 | ID requested | |
⏵ 1 | 2022-02-24 14:26:41 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: co infection, ubiquitin, influenza A virus, Streptococcus pneumoniae D39, A549 |
Contact List
Doerte Becher |
contact affiliation | University of Greifswald, Center for Functional Genomics of Microbes, Institute of Microbiology, Department of Microbial Proteomics, Felix Hausdorff Str. 8, 17489 Greifswald, Germany |
contact email | doerte.becher@uni-greifswald.de |
lab head | |
Thomas Sura |
contact affiliation | University of Greifswald, Center for Functional Genomics of Microbes, Institute for Microbiology, Department of Microbial Proteomics |
contact email | thomas.sura@uni-greifswald.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD028465
- Label: PRIDE project
- Name: Streptococcus pneumoniae and IAV co infection induces altered polyubiquitination in A549 cells