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PXD028465

PXD028465 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleStreptococcus pneumoniae and IAV co infection induces altered polyubiquitination in A549 cells
DescriptionEpithelial cells are the first line of defense within the lung. Disruption of the epithelial barrier by pathogens enables the systematic dissemination of bacteria or viruses within the host, leading to severe diseases with fatal outcomes. Thus, the lung epithelium can be damaged by seasonal and pandemic influenza A viruses. Influenza A virus infection induced dysregulation of the immune system is beneficial for the dissemination of bacteria to the lower respiratory tract, causing bacterial and viral co-infection. Host cells regulate protein homeostasis and the response to different stimuli, for instance pathogen infections, by post translational modification of proteins. Aside from protein phosphorylation, ubiquitination of proteins is an essential regulatory tool in virtually every cellular process, such as protein homeostasis, the host immune response, cell morphology, and in clearing of cytosolic pathogens. Here, we analyzed the proteome and ubiquitinome of A549 cells in response to Streptococcus pneumoniae D39 Δcps and influenza A virus H1N1 as well as bacterial and viral co-infection. Pneumococcal infection induced alterations in the ubiquitination of proteins involved in the organization of the actin cytoskeleton and Rho GTPases, but had minor effects on the abundance of host proteins. H1N1 infection is reflected by an anti-viral state of A549 cells. Finally, co infection resembled the imprints of both infecting pathogens with a minor increase in the observed alterations in protein and ubiquitination abundance.
HostingRepositoryPRIDE
AnnounceDate2022-02-24
AnnouncementXMLSubmission_2022-02-24_14:26:41.504.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterThomas Sura
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-09-14 15:37:04ID requested
12022-02-24 14:26:41announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: co infection, ubiquitin, influenza A virus, Streptococcus pneumoniae D39, A549
Contact List
Doerte Becher
contact affiliationUniversity of Greifswald, Center for Functional Genomics of Microbes, Institute of Microbiology, Department of Microbial Proteomics, Felix Hausdorff Str. 8, 17489 Greifswald, Germany
contact emaildoerte.becher@uni-greifswald.de
lab head
Thomas Sura
contact affiliationUniversity of Greifswald, Center for Functional Genomics of Microbes, Institute for Microbiology, Department of Microbial Proteomics
contact emailthomas.sura@uni-greifswald.de
dataset submitter
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