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PXD028463

PXD028463 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleBasolateral Protein Scribble Provides Phosphatase PP1 to a Signaling Network Maintaining Apicobasal Polarity
DescriptionScribble, a member of the LAP protein family, contributes to the apicobasal polarity (ABP) of epithelial cells. The LAP unique region (LUR) of these proteins is essential for ABP function, and includes a highly conserved Leucine Reach Repeat (LRR) domain. Here we study how the LRR domain of Scribble maintains ABP. We show that its concave surface participates in three types of mutually exclusive interactions 1. homodimerization serving as an auto-inhibitory mechanism, 2. interactions with a diverse set of polarity proteins, such as Llgl1, Llgl2, EPB41L2 and EPB41L5, which produce distinct multiprotein complexes, and 3. a direct interaction with the protein phosphatase, PP1, which forms a dimeric LRR domain-PP1 complex. Our results suggest that the latter complex maintains PP1 in the basolateral cell cortex in close proximity to PP1 targets. Such organization generates a dynamic network where PP1 could be immediately dispatched from the Scribble-PP1 complex to particular protein ligands. This mechanism for controlling dephosphorylation kinetics of phosphorylated proteins could be universal for all members of the LAP protein family, which includes Erbin, Lano, and the neuron-specific protein, Densin-180.
HostingRepositoryMassIVE
AnnounceDate2021-09-14
AnnouncementXMLSubmission_2021-09-14_13:23:26.937.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterIndrajyoti Indra
SpeciesList scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentLTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-09-14 12:19:19ID requested
12021-09-14 13:23:27announced
Publication List
no publication
Keyword List
submitter keyword: Scribble, Apicobasal Polarity, Phosphatase PP1
Contact List
Sergey M Troyanovsky
contact affiliationNorthwestern University
contact emails-troyanovsky@northwestern.edu
lab head
Indrajyoti Indra
contact affiliationNorthwestern University
contact emailindrajyoti.indra@northwestern.edu
dataset submitter
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