PXD028462

PXD028462 is an original dataset announced via ProteomeXchange.

Title	Identifying global and mtCU independent MICU1 interactome utilizing BioID2 based proximity labeling and screening
Description	MICU1 is a Ca2+-binding protein that regulates the mitochondrial Ca2+ uniporter channel (mtCU ) and mitochondrial Ca2+ (mCa2+) uptake. MICU1 knockout mice display perinatal lethality and disorganized mitochondrial architecture. These phenotypes are distinct from other mtCU loss-of-function models and thus are not explained by changes in mCa2+ content. Utilizing multiple proteomic approaches, we found that MICU1 localized to mitochondrial complexes lacking MCU, suggesting that MICU1 has cellular functions independent of mCa2+ uptake. The overall aim of the current project is to identify the global and mtCU independent MICU1 interactome to characterize the MCU independent functions of the MICU1.
HostingRepository	PRIDE
AnnounceDate	2024-01-26
AnnouncementXML	Submission_2024-01-26_05:50:48.313.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dhanendra Tomar
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	biotinylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-14 09:40:35	ID requested
⏵ 1	2024-01-26 05:50:49	announced

10.1126/scisignal.abi8948;

Tomar D, Thomas M, Garbincius JF, Kolmetzky DW, Salik O, Jadiya P, Joseph SK, Carpenter AC, Hajn, ó, czky G, Elrod JW, uniporter channel. Sci Signal, 16(782):eabi8948(2023) [pubmed]

submitter keyword: bioid2,mitochondria, calcium, proximity-labelling, mcu, micu1

John W. Elrod
contact affiliation	Center for Translational Medicine, Lewis Katz School of Medicine at Temple University, 3500 N Broad St, MERB 949, Philadelphia, PA, 19140, USA.
contact email	elrod@temple.edu
lab head
Dhanendra Tomar
contact affiliation	Lewis Katz School of Medicine at Temple University
contact email	dhtomar@temple.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD028462

PRIDE project URI

• PRIDE
  1. PXD028462
     1. Label: PRIDE project
     2. Name: Identifying global and mtCU independent MICU1 interactome utilizing BioID2 based proximity labeling and screening