PXD028461

PXD028461 is an original dataset announced via ProteomeXchange.

Title	Phosphorylation Mapping of Atg13 in Response to Atg1 Activity and Rapamycin Treatment in Budding Yeast
Description	We performed phosphorylation site mapping on of budding yeast Atg13 applying a quantitative mass spectrometry-based proteomics approach based on stable isotope labeling with amino acids in cell culture (SILAC). We determined changes in the Atg13 phosphorylation pattern in response of inhibition of kinase Atg1 and starvation conditions (using rapamycin), respectively. To do so, Atg13 was tandem purified via a histidine-biotin (HB) tag from cells growing at the respective experimental condition. Different stimulus-dependent PTM profiles of Atg13 have been identified.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:24:06.546.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wolfgang Reiter
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	phosphorylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-14 09:34:59	ID requested
1	2024-01-20 11:32:17	announced
⏵ 2	2024-10-22 06:24:07	announced	2024-10-22: Updated project metadata.

Bhattacharya A, Torggler R, Reiter W, Romanov N, Licheva M, Ciftci A, Mari M, Kolb L, Kaiser D, Reggiori F, Ammerer G, Hollenstein DM, Kraft C, Decoding the function of Atg13 phosphorylation reveals a role of Atg11 in bulk autophagy initiation. EMBO Rep, 25(2):813-831(2024) [pubmed]

10.1038/s44319-023-00055-9;

submitter keyword: Phosphorylation, Autophagy, Saccharomyces cerevisiae, Quantitative Proteomics

Claudine Kraft
contact affiliation	Institute of Biochemistry and Molecular Biology, Albert-Ludwigs-University of Freiburg
contact email	claudine.kraft@biochemie.uni-freiburg.de
lab head
Wolfgang Reiter
contact affiliation	Mass Spectrometry Facility, Max Perutz Labs Vienna, University of Vienna, Vienna Biocenter, Dr.-Bohr-Gasse 7, 1030 Vienna
contact email	wolfgang.l.reiter@univie.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD028461

PRIDE project URI

• PRIDE
  1. PXD028461
     1. Label: PRIDE project
     2. Name: Phosphorylation Mapping of Atg13 in Response to Atg1 Activity and Rapamycin Treatment in Budding Yeast