PXD028457

PXD028457 is an original dataset announced via ProteomeXchange.

Title	Nuclear pore protein complex-crosslink
Description	The nuclei were isolated from HeLa cells with nuclear extraction reagents (Thermo, NE-PER™) and suspended in 1 X PBS buffer (pH 7.4). The suspension of intact nuclei was crosslinked by adding 5 mM BSP (chemical structure shown in Figure S1, with the maximum Cα-Cα distance restraints of 28 Å ) every 20 min for 3 times at room temperature and quenched with 50 mM ammonium bicarbonate. To further improve the identification coverage of NRPC, the cross-linker of 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methyl morpholinium chloride (DMTMM) was used, combining with the amino-reactive BSP for the crosslinking of nuclear envelopes (NEs) isolated from HeLa cells with commercial kit (invent, NE-013™), respectively.
HostingRepository	iProX
AnnounceDate	2021-09-14
AnnouncementXML	Submission_2021-09-13_23:48:30.251.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bowen Zhong
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-13 23:47:10	ID requested
⏵ 1	2021-09-13 23:48:30	announced

Dataset with its publication pending

submitter keyword: Nuclear pore protein, Crosslink, Protein structure

Qun Zhao
contact affiliation	Dalian Institute Of Chemical Physics
contact email	zhaoqun@dicp.ac.cn
lab head
Bowen Zhong
contact affiliation	Dalian Institute Of Chemical Physics
contact email	zhongbowen1810@dicp.ac.cn
dataset submitter

iProX dataset URI