PXD028417

PXD028417 is an original dataset announced via ProteomeXchange.

Title	Cholangiocyte organoid culture in hydrogels from decellularized liver tissue
Description	Culture of human cholangiocyte organoids in hydrogels derived from healthy liver extracellular matrix (LECM) extracts prepared from decellularized human livers are evaluated in an effort to establish a platform for production of cholangiocyte organoids for clinical regenerative applications. Human intrahepatic cholangiocyte organoids (ICO) grown in hydrogels made from LECM are compared those grown in mouse tumor derived basement membrane extracts (BME). Culture was performed with amino acids labeled with stable heavy isotopes to enable separation of ECM from the hydrogels from that of the produced by the cells with mass spectrometry (MS). MS data were used to evaluate the protein production of ICO comparing the different hydrogel substrates. The study also contains evaluation of the properties of the hydrogel substrates and focuses on expansion and differentiation of the ICO.
HostingRepository	PRIDE
AnnounceDate	2022-04-13
AnnouncementXML	Submission_2022-04-13_03:43:52.765.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD028417
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Oskar Rosmark
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	4-hydroxy-L-proline; acetylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-09 09:01:48	ID requested
⏵ 1	2022-04-13 03:43:53	announced

Willemse J, van Tienderen G, van Hengel E, Schurink I, van der Ven D, Kan Y, de Ruiter P, Rosmark O, Westergren-Thorsson G G, Schneeberger K, van der Eerden B, Roest H, Spee B, van der Laan L, de Jonge J, Verstegen M, Hydrogels derived from decellularized liver tissue support the growth and differentiation of cholangiocyte organoids. Biomaterials, 284():121473(2022) [pubmed]

submitter keyword: Human, liver, organoid, cholangiocyte, hydrogel, regeneration

Prof. Luc van der Laan
contact affiliation	Department of Surgery, Erasmus MC-University Medical Center, Rotterdam, The Netherlands.
contact email	l.vanderlaan@erasmusmc.nl
lab head
Oskar Rosmark
contact affiliation	Lung Biology, Department Experimental Medical Science, Lund University, Lund, Sweden.
contact email	oskar.rosmark@med.lu.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD028417

PRIDE project URI

• PRIDE
  1. PXD028417
     1. Label: PRIDE project
     2. Name: Cholangiocyte organoid culture in hydrogels from decellularized liver tissue