PXD028400

PXD028400 is an original dataset announced via ProteomeXchange.

Title	Proteomic study of triple negative breast cancer cell lines at different tumorigenic stages reveals a divergent reprogramming metabolism
Description	Breast cancer is the leading type of cancer. The triple negative is the most aggressive type of breast cancer, as it is resistant to several treatments as hormone based, for example. Cell metabolism is altered in cancer cells as they favor anaerobic pathways to produce energy in a faster pace and in accordance with the hypoxic environments found inside tumor mass, which is known as the Warburg effect. In this study, we sought to investigate the molecular mechanisms behind the different cell phenotypes during tumorigenesis. To pursue this goal, we employed proteomics in four types of cells; the non-tumorigenic epithelial cell MCF-10A, two triple negative breast cancer originated from primary tumor sites (MGSO-3 and MACL-1), and the metastatic tumor cell line MDA-231-MD. Our proteomic data shown that most proteins associated with the TCA cycle and oxidative phosphorylation (aerobic metabolic phase) were upregulated in primary cells while downregulated in metastatic cell, in line with a negative regulation of the Warburg effect for the primarily cells. We also shown that these cells have lower mitochondria density and upregulation of pro-apoptotic proteins when compared with metastatic cells. Molecular differences highlighted in this study may assist the scientific community in developing new protocols to treat the triple negative breast cancer.
HostingRepository	PRIDE
AnnounceDate	2022-06-07
AnnouncementXML	Submission_2022-06-07_12:15:08.449.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thiago Verano-Braga
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-09 08:36:02	ID requested
⏵ 1	2022-06-07 12:15:09	announced

Braga FHG, G, ó, mez-Mendoza DP, Lemos RP, Rodrigues-Ribeiro L, Ra, í, ssa-Oliveira B, Rodrigues ALP, Gorshkov V, Kjeldsen F, Cruz JS, Verano-Braga T, Proteomic analysis reveals stage-specific reprogramed metabolism for the primary breast cancer cell lines MGSO-3 and MACL-1. Proteomics, 22(17):e2200095(2022) [pubmed]

ProteomeXchange project tag: Cancer (B/D-HPP), Biology/Disease-Driven Human Proteome Project (B/D-HPP), Human Proteome Project

submitter keyword: Breast Cancer, Proteomics, TMT

Thiago Verano Braga
contact affiliation	Department of Physiology and Biophysics, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte-MG, Brazil.
contact email	tvbraga@icb.ufmg.br
lab head
Thiago Verano-Braga
contact affiliation	Federal University of Minas Gerais
contact email	npf.ufmg@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/06/PXD028400

PRIDE project URI

• PRIDE
  1. PXD028400
     1. Label: PRIDE project
     2. Name: Proteomic study of triple negative breast cancer cell lines at different tumorigenic stages reveals a divergent reprogramming metabolism