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PXD028392

PXD028392 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMultiomics Profiling of Alzheimer’s Disease Serum for the Identification of Autoantibody Biomarkers
DescriptionNew biomarkers of Alzheimer’s Disease (AD) with diagnostic value in preclinical and prodromal stages are urgently needed. AD-related serum autoantibodies are potential candidate biomarkers. Here, we aimed at identifying AD-related serum autoantibodies using protein microarrays and mass spectrometry-based methods. To this end, an untargeted complementary screening using high-density (42,100 antigens) and low-density (384 antigens) planar protein-epitope signature tag (PrEST) arrays and an immunoprecipitation protocol coupled to mass spectrometry analysis were used for serum autoantibody profiling. From the untargeted screening phase, 377 antigens corresponding to 338 proteins were selected for validation. Out of them, IVD, CYFIP1 and ADD2 seroreactivity was validated using 128 sera from AD patients and controls by PrEST-suspension beads arrays, and ELISA or luminescence Halotag-based bead immunoassay using full-length recombinant proteins. Importantly, IVD, CYFIP1, and ADD2 showed in combination noticeable AD diagnostic ability. Moreover, IVD protein abundance in the prefrontal cortex was significantly two-fold higher in AD patients than in controls by WB and immunohistochemistry, whereas CYFIP1 and ADD2 were significantly down-regulated in AD patients. The panel of AD-related autoantigens identified by a comprehensive multiomics approach may provide new insights of the disease and should help in the blood-based diagnosis of Alzheimer’s disease.
HostingRepositoryPRIDE
AnnounceDate2021-12-30
AnnouncementXMLSubmission_2021-12-30_14:06:46.651.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterRodrigo Barderas
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListphosphorylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-09-09 08:35:10ID requested
12021-12-30 14:06:47announced
Publication List
San Segundo-Acosta P, Montero-Calle A, Jernbom-Falk A, Alonso-Navarro M, Pin E, Andersson E, Hellstr, ö, m C, S, á, nchez-Mart, í, nez M, R, á, bano A, Sol, í, s-Fern, á, ndez G, Pel, á, ez-Garc, í, a A, Mart, í, nez-Useros J, Fern, á, ndez-Ace, ñ, ero MJ, M, å, nberg A, Nilsson P, Barderas R, Multiomics Profiling of Alzheimer's Disease Serum for the Identification of Autoantibody Biomarkers. J Proteome Res, 20(11):5115-5130(2021) [pubmed]
Keyword List
submitter keyword: autoantibodies, autoantigens, Alzheimer's disease, protein microarrays, mass spectrometry, PrEST microarrays
Contact List
Rodrigo Barderas
contact affiliationFunctional Proteomics Unit Chronic Disease Programme Instituto de Salud Carlos III
contact emailr.barderasm@isciii.es
lab head
Rodrigo Barderas
contact affiliationInstituto de Salud Carlos III
contact emailr.barderasm@isciii.es
dataset submitter
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Dataset FTP location
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