PXD028392

PXD028392 is an original dataset announced via ProteomeXchange.

Title	Multiomics Profiling of Alzheimer’s Disease Serum for the Identification of Autoantibody Biomarkers
Description	New biomarkers of Alzheimer’s Disease (AD) with diagnostic value in preclinical and prodromal stages are urgently needed. AD-related serum autoantibodies are potential candidate biomarkers. Here, we aimed at identifying AD-related serum autoantibodies using protein microarrays and mass spectrometry-based methods. To this end, an untargeted complementary screening using high-density (42,100 antigens) and low-density (384 antigens) planar protein-epitope signature tag (PrEST) arrays and an immunoprecipitation protocol coupled to mass spectrometry analysis were used for serum autoantibody profiling. From the untargeted screening phase, 377 antigens corresponding to 338 proteins were selected for validation. Out of them, IVD, CYFIP1 and ADD2 seroreactivity was validated using 128 sera from AD patients and controls by PrEST-suspension beads arrays, and ELISA or luminescence Halotag-based bead immunoassay using full-length recombinant proteins. Importantly, IVD, CYFIP1, and ADD2 showed in combination noticeable AD diagnostic ability. Moreover, IVD protein abundance in the prefrontal cortex was significantly two-fold higher in AD patients than in controls by WB and immunohistochemistry, whereas CYFIP1 and ADD2 were significantly down-regulated in AD patients. The panel of AD-related autoantigens identified by a comprehensive multiomics approach may provide new insights of the disease and should help in the blood-based diagnosis of Alzheimer’s disease.
HostingRepository	PRIDE
AnnounceDate	2021-12-30
AnnouncementXML	Submission_2021-12-30_14:06:46.651.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rodrigo Barderas
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-09 08:35:10	ID requested
⏵ 1	2021-12-30 14:06:47	announced

San Segundo-Acosta P, Montero-Calle A, Jernbom-Falk A, Alonso-Navarro M, Pin E, Andersson E, Hellstr, ö, m C, S, á, nchez-Mart, í, nez M, R, á, bano A, Sol, í, s-Fern, á, ndez G, Pel, á, ez-Garc, í, a A, Mart, í, nez-Useros J, Fern, á, ndez-Ace, ñ, ero MJ, M, å, nberg A, Nilsson P, Barderas R, Multiomics Profiling of Alzheimer's Disease Serum for the Identification of Autoantibody Biomarkers. J Proteome Res, 20(11):5115-5130(2021) [pubmed]

submitter keyword: autoantibodies, autoantigens, Alzheimer's disease, protein microarrays, mass spectrometry, PrEST microarrays

Rodrigo Barderas
contact affiliation	Functional Proteomics Unit Chronic Disease Programme Instituto de Salud Carlos III
contact email	r.barderasm@isciii.es
lab head
Rodrigo Barderas
contact affiliation	Instituto de Salud Carlos III
contact email	r.barderasm@isciii.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/12/PXD028392

PRIDE project URI

• PRIDE
  1. PXD028392
     1. Label: PRIDE project
     2. Name: Multiomics Profiling of Alzheimer’s Disease Serum for the Identification of Autoantibody Biomarkers