PXD028372

PXD028372 is an original dataset announced via ProteomeXchange.

Title	Characterization of a novel membrane complex required for docking and regulated exocytosis of lysosome-related organelles in Tetrahymena thermophila
Description	In the ciliate Tetrahymena thermophila, lysosome-related organelles called mucocysts accumulate at the cell periphery where they secrete their contents in response to extracellular events, a phenomenon termed regulated exocytosis. A forward genetic approach uncovered MDL1 (Mucocyst Discharge with a LamG domain) as essential gene for regulated exocytosis of mucocysts. The encoded protein, Mdl1p, is a 66 kD membrane-associated glycoprotein that localizes to mucocysts, and specifically to a tip domain that contacts the plasma membrane when the mucocyst is docked. Immunoisolation by affinity capture using Mdl1p-FLAG as bait, described here, revealed that Mdl1p forms a complex with 9 other proteins, all of them novel and largely restricted to Alveolates.
HostingRepository	PRIDE
AnnounceDate	2022-04-06
AnnouncementXML	Submission_2022-04-06_05:02:57.249.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Martin Zoltner
SpeciesList	scientific name: Tetrahymena thermophila CU428; NCBI TaxID: 452467;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-09 08:30:12	ID requested
⏵ 1	2022-04-06 05:02:57	announced

Dataset with its publication pending

submitter keyword: Tetrahymena, exocytosis, mucocysts, lysosome, ciliate, MDL1

Dr. Martin Zoltner
contact affiliation	Head of Drug Discovery and Evaluation Center for Research of Pathogenicity and Virulence of Parasites Charles University in Prague, BIOCEV Průmyslová 595 252 50 Vestec Czech Republic
contact email	zoltnerm@natur.cuni.cz
lab head
Martin Zoltner
contact affiliation	Division of Biological Chemistry & Drug Discovery School of Life Sciences University of Dundee Dundee DD1 5EH
contact email	m.zoltner@dundee.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD028372

PRIDE project URI

• PRIDE
  1. PXD028372
     1. Label: PRIDE project
     2. Name: Characterization of a novel membrane complex required for docking and regulated exocytosis of lysosome-related organelles in Tetrahymena thermophila