PXD028368

PXD028368 is an original dataset announced via ProteomeXchange.

Title	Profiling of IgG N-glycome in gastric cancer by MALDI-TOF-MS
Description	Background: Alterations in IgG glycosylation have been observed in many inflammatory activities and diseases progression, uncovering IgG glycome contributes to deciphering the molecular mechanism of human diseases. Gastric cancer (GC) associated IgG glycosylation was rarely investigated.Methods: We comprehensively characterized IgG N-glycome profile in GC by high-throughput orthogonal mass spectrometry assay, and analyzed the difference of glycosylation between controls and GC patients through multiple statistical tools. Bioinformatics analysis was performed to study the candidate glycogens and their biological functions involved in GC. Results: GC associates with decrease in galactosylation and sialylation of IgG, and increase in bisection and agalactosylation of IgG.Bioinformatics analysis verified the differential expression of significantly changed IgG glycan associated glycogens, indicating the crucial role of IgG glycosylation in the pathogenesis of GC.Conclusions: This study demonstrates the potential diagnostic merits of specific IgG glycans for early detection of GC, as well as revealing molecular mechanism in the development of this cancer.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_08:48:25.325.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Si Liu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	4800 Proteomics Analyzer

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-09 08:29:47	ID requested
⏵ 1	2025-05-06 08:48:26	announced

10.1186/s12885-025-13616-z;

Xu T, Huang J, Lin J, Liu Y, Wang Y, Shen W, He J, Chen S, Zhu X, Que Y, Hu M, Chen Y, Cheng L, He H, Liu X, Liu S, Site-specific immunoglobulin G N-glycosylation is associated with gastric cancer progression. BMC Cancer, 25(1):217(2025) [pubmed]

submitter keyword: Human serum, IgG, gastric cancer, N-glycome, MALDI-MS

Si Liu
contact affiliation	Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, N0. 1037 Luoyudong Road, Hongshan District, Wuhan 430074
contact email	xliu@mail.hust.edu.cn
lab head
Si Liu
contact affiliation	Huazhong University of Science and Technology
contact email	siliu2019@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD028368
     1. Label: PRIDE project
     2. Name: Profiling of IgG N-glycome in gastric cancer by MALDI-TOF-MS