PXD028365

PXD028365 is an original dataset announced via ProteomeXchange.

Title	2′ ,3′-cAMP treatment mimics abiotic stress response
Description	For many years, 2′,3′-cyclic adenosine monophosphate (2′,3′-cAMP), a positional isomer of the second messenger 3′,5′-cAMP, has not received enough attention. Recent studies have reported that 2′,3′-cAMP exists in plants and might be involved in stress signaling because 1) its level increases upon wounding and 2) it was shown to participate in stress granule formation. Although 2′,3′-cAMP is known as RNA-degradation product, the effect of its accumulation in the cell remains unknown. Here, we unprecedentedly evaluate responses at the transcriptome, metabolome, and proteome levels to the accumulation of 2′,3′-cAMP in Arabidopsis plants. Data revealed that 2′,3′-cAMP is metabolized into adenosine, suggesting that a well-known cyclic nucleotide - adenosine pathway from human cells might exist also in plants. However, the control transcriptomic data indicated that, despite fewer overlaps, responses to 2′,3′-cAMP and to adenosine differ. Analysis of the transcriptome and proteome in response to 2′,3′-cAMP showed similar changes, known as signatures for abiotic stress response. This is further supported by the fact that adding to the role in facilitating of stress granules (SGs) formation, 2′,3′-cAMP induces changes at the level of proteins known as key components of SGs and can also induce movement of processing bodies (PBs) in the cell.
HostingRepository	PRIDE
AnnounceDate	2022-05-19
AnnouncementXML	Submission_2022-05-19_10:35:10.959.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michał Górka
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-09 08:29:32	ID requested
⏵ 1	2022-05-19 10:35:11	announced

Chodasiewicz M, Kerber O, Gorka M, Moreno JC, Maruri-Lopez I, Minen RI, Sampathkumar A, Nelson ADL, Skirycz A, 2',3'-cAMP treatment mimics the stress molecular response in Arabidopsis thaliana. Plant Physiol, 188(4):1966-1978(2022) [pubmed]

submitter keyword: 2′,3′-cAMP, abiotic stress, processing bodies, stress response, stress granules

Aleksandra Skirycz
contact affiliation	Max Planck Institute of Molecular Plant Physiology, 14476 Potsdam, Germany
contact email	skirycz@mpimp-golm.mpg.de
lab head
Michał Górka
contact affiliation	Celon Pharma
contact email	michal.gorka.ofc@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD028365
     1. Label: PRIDE project
     2. Name: 2′ ,3′-cAMP treatment mimics abiotic stress response