PXD028358

PXD028358 is an original dataset announced via ProteomeXchange.

Title	Proteotoxicity caused by perturbed protein complexes underlies hybrid incompatibility
Description	Dobzhansky-Muller incompatibilities represent a major driver of reproductive isolation between species. They are caused when two or more interacting components encoded by alleles from different species cannot function properly when mixed. At incipient stages of speciation, complex incompatibilities involving multiple genetic loci with weak effects are frequently observed, but the underlying mechanisms remain elusive. We observed perturbed proteostasis leading to compromised mitosis and meiosis in Saccharomyces cerevisiae hybrid lines carrying one or two chromosomes from Saccharomyces bayanus var uvarum. Levels of proteotoxicity are correlated with the number of protein complexes on replaced chromosomes and can be alleviated or aggravated, respectively, by up- or down-regulating the ubiquitin-proteasomal degradation machinery. Using proteomic approaches, we detect destabilized multi-protein complexes in a hybrid line. However, hybrid fitness can be significantly improved by rescuing small ribosomal subunits, a primary destabilized complex. Our findings reveal the general role of impaired protein complex assembly in complex incompatibilities.
HostingRepository	PRIDE
AnnounceDate	2022-07-06
AnnouncementXML	Submission_2022-07-05_16:43:17.074.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yi-Yun Chen
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-09 08:28:58	ID requested
⏵ 1	2022-07-05 16:43:17	announced

Dataset with its publication pending

submitter keyword: speciation, genetic incompatibility, proteostasis, protein complex, proteotoxicity, epistasis

Jun-Yi Leu
contact affiliation	Institute of Molecular Biology, Academia Sinica, Taipei 11529, Taiwan
contact email	jleu@imb.sinica.edu.tw
lab head
Yi-Yun Chen
contact affiliation	Institute of Biological Chemistry/Academia Sinica
contact email	badmaru@gate.sinica.edu.tw
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD028358
     1. Label: PRIDE project
     2. Name: Proteotoxicity caused by perturbed protein complexes underlies hybrid incompatibility