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PXD028358 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteotoxicity caused by perturbed protein complexes underlies hybrid incompatibility
DescriptionDobzhansky-Muller incompatibilities represent a major driver of reproductive isolation between species. They are caused when two or more interacting components encoded by alleles from different species cannot function properly when mixed. At incipient stages of speciation, complex incompatibilities involving multiple genetic loci with weak effects are frequently observed, but the underlying mechanisms remain elusive. We observed perturbed proteostasis leading to compromised mitosis and meiosis in Saccharomyces cerevisiae hybrid lines carrying one or two chromosomes from Saccharomyces bayanus var uvarum. Levels of proteotoxicity are correlated with the number of protein complexes on replaced chromosomes and can be alleviated or aggravated, respectively, by up- or down-regulating the ubiquitin-proteasomal degradation machinery. Using proteomic approaches, we detect destabilized multi-protein complexes in a hybrid line. However, hybrid fitness can be significantly improved by rescuing small ribosomal subunits, a primary destabilized complex. Our findings reveal the general role of impaired protein complex assembly in complex incompatibilities.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterYi-Yun Chen
SpeciesList scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationListiodoacetamide derivatized residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-09-09 08:28:58ID requested
12022-07-05 16:43:17announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: speciation, genetic incompatibility, proteostasis, protein complex, proteotoxicity, epistasis
Contact List
Jun-Yi Leu
contact affiliationInstitute of Molecular Biology, Academia Sinica, Taipei 11529, Taiwan
contact emailjleu@imb.sinica.edu.tw
lab head
Yi-Yun Chen
contact affiliationInstitute of Biological Chemistry/Academia Sinica
contact emailbadmaru@gate.sinica.edu.tw
dataset submitter
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