Hypsizygus marmoreus is one of the most popular industrialized cultivation mushrooms in East Asia, including China, Korea and Japan. It draws our attention due to its desirable, mildly sweet nutty flavor, unique texture and medicinal properties. As a result, the number of H. marmoreus cultivation factories increased gradually in different regions of the world, especially in China. H. marmoreus is the third wildly cultivated industrialized cultivated mushroom in China now. However, its development process is still not very clear. In this study, we use proteomics analysis to investigate the mechanism involved in development of Hypsizygus marmoreus.