PXD028347

PXD028347 is an original dataset announced via ProteomeXchange.

Title	Inactivation of the response regulator AgrA has a pleiotropic effect on biofilm formation, pathogenesis and stress response in Staphylococcus lugdunensis
Description	Staphylococcus lugdunensis is a coagulase-negative Staphylococcus that emerges as an important opportunistic pathogen. However, little is known about the regulation underlying the transition from commensal to virulent state. Based on knowledge of S. aureus virulence, we suspected that the agr quorum sensing system may be an important determinant for the pathogenicity of S. lugdunensis. We investigated the functions of the transcriptional regulator AgrA using the agrA deletion mutant. AgrA played a role in cell pigmentation: ∆argA mutant colonies were white while the parental strains were slightly yellow. Compared to the wild-type strain, the ∆argA mutant was affected in its ability to form biofilm and was less able to survive in mice macrophages. Moreover, the growth of ∆agrA was significantly reduced by the addition of 10 % NaCl or 0.4 mM H2O2 and its survival after 2 h in presence of 1 mM H2O2 was more than 10-fold reduced. To explore the mechanisms involved beyond these phenotypes, the ∆agrA proteome and transcriptome were characterized by mass spectrometry and RNA-Seq. We found that AgrA controlled several virulence factors as well as stress-response factors, which are well correlated with the reduced resistance of the ∆agrA mutant to osmotic and oxidative stresses. These results were not the consequence of the deregulation of RNAIII of the agr system, since no phenotype or alteration of the proteomic profile has been observed for the ∆RNAIII mutant. Altogether, our results highlighted that the AgrA regulator of S. lugdunensis played a key role in its ability to become pathogenic.
HostingRepository	PRIDE
AnnounceDate	2022-02-22
AnnouncementXML	Submission_2022-02-22_04:10:27.500.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	benoit bernay
SpeciesList	scientific name: Staphylococcus lugdunensis; NCBI TaxID: 28035;
ModificationList	carbamoylated residue; S-carboxamidoethyl-L-cysteine; acetylated residue; monohydroxylated residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-09 08:27:53	ID requested
⏵ 1	2022-02-22 04:10:28	announced

Aubourg M, Pottier M, L, é, on A, Bernay B, Dhalluin A, Cacaci M, Torelli R, Ledormand P, Martini C, Sanguinetti M, Auzou M, Gravey F, Giard JC, Inactivation of the Response Regulator AgrA Has a Pleiotropic Effect on Biofilm Formation, Pathogenesis and Stress Response in Staphylococcus lugdunensis. Microbiol Spectr, 10(1):e0159821(2022) [pubmed]

submitter keyword: Staphylococcus lugdunensis, AgrA, transcriptional regulation, agr system

Benoit Bernay
contact affiliation	Plateforme Proteogen SF ICORE 4206, US EMerode Université de Caen Normandie Esplanade de la paix, 14032 Caen cedex
contact email	benoit.bernay@unicaen.fr
lab head
benoit bernay
contact affiliation	Proteogen platform
contact email	benoit.bernay@unicaen.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD028347
     1. Label: PRIDE project
     2. Name: Inactivation of the response regulator AgrA has a pleiotropic effect on biofilm formation, pathogenesis and stress response in Staphylococcus lugdunensis