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PXD028343

PXD028343 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePROTEOMIC PROFILING OF AMNIOS FOR PRETERM BIRTH BIOMARKER DISCOVERY
DescriptionSpontaneous preterm birth (PTB) complicates about 12% of pregnancies worldwide, remaining the main cause of neonatal morbidity and mortality. A consistent number of PTB are caused by microbial-induced preterm labor, mediated by an inflammatory process, that threatens both maternal and newborn health. In search for novel predictive biomarkers of PTB and PROM, and to improve understanding of infection related PTB, we performed a proteomic analysis of amnions from premature newborns. Samples were studied by Orbitrap mass spectrometry and bioinformatics analyses. A total of 6352 proteins were identified. A ranked core of 159 proteins maximized the discrimination between the different clinical stratification groups of amnions allowing to distinguish FIR 0 from FIR 3, stratified in function of MIR grade, among which Matrix metallopeptidase 9 (MMP-9) was the top differentially expressed protein. Gene Ontology enrichment analysis of the core proteins showed significant changes in the biological pathways associated to inflammation and regulation of immune and infection response. Data suggest that the conditions determining PTB would be a transversal event, secondary to the maternal inflammatory response causing a breakdown in fetal-maternal tolerance, with fetal inflammation being severe than maternal one. We also highlight matrix metallopeptidase-9 as a potential amnion-associated predictive biomarker of PTB that can be assayed in the maternal serum, for future investigation.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:52:39.205.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMartina Bartolucci
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListacetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-09-09 08:27:35ID requested
12021-12-06 02:46:03announced
22023-11-14 08:52:39announced2023-11-14: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: proteomics, inflammation,Amnios, mass spectrometry, preterm birth, biomarker
Contact List
Andrea Petretto
contact affiliationIRCCS Istituto Giannina Gaslini
contact emaila.petretto@gmail.com
lab head
Martina Bartolucci
contact affiliationIRCCS Gaslini
contact emailsmartibartolucci@gmail.com
dataset submitter
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Dataset FTP location
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