PXD028332

PXD028332 is an original dataset announced via ProteomeXchange.

Title	Antibiotic-Chemoattractants Enhance Neutrophil Clearance of Staphylococcus aureus
Description	S. aureus is a deadly pathogen due to its abilities to readily develop antibiotic resistance and evade our immune system. Antibiotic resistance in S. aureus is associated with reduced levels of neutrophil recruitment, which is a vital step in triggering an immune response to resolve infection. In this work, we report enhanced antibiotic agents that act as potential dual-function antibiotic-chemoattractants, enabling augmented neutrophil recruitment to S. aureus along with direct killing. Our agents exploit formylated peptides as chemoattractants for neutrophil recruitment, which is combined with the targeted binding of vancomycin to bacteria that generates a chemoattractant gradient for neutrophil recruitment. The combination of in vitro assays, cellular assays, infection-on-a-chip and in vivo mouse models, determined that these antibiotic-chemoattractants improve the recruitment, engulfment and killing of S. aureus by neutrophils. Furthermore, optimizing the fPep sequence can play an important role in the enhancement of neutrophil activity through differential activation of formyl peptide receptors. This offers an alternate approach in antibiotic development to overcome the threat of antibiotic resistance in the clinic.
HostingRepository	PRIDE
AnnounceDate	2021-09-22
AnnouncementXML	Submission_2021-12-15_08:28:54.826.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ralf Schittenhelm
SpeciesList	scientific name: Staphylococcus aureus; NCBI TaxID: 1280;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-09 08:26:32	ID requested
1	2021-09-21 17:55:53	announced
⏵ 2	2021-12-15 08:28:55	announced	2021-12-15: Updated publication reference for PubMed record(s): 34697316.

Payne JAE, Tailhades J, Ellett F, Kostoulias X, Fulcher AJ, Fu T, Leung R, Louch S, Tran A, Weber SA, Schittenhelm RB, Lieschke GJ, Qin CH, Irima D, Peleg AY, Cryle MJ, Antibiotic-chemoattractants enhance neutrophil clearance of Staphylococcus aureus. Nat Commun, 12(1):6157(2021) [pubmed]

submitter keyword: immunotherapeutic, superbug, antibiotic, neutrophil, chemotaxis, innate immunity

Max J Cryle
contact affiliation	Infection and Immunity Program, Monash Biomedicine Discovery Institute, Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria, 3800 Australia
contact email	max.cryle@monash.edu
lab head
Ralf Schittenhelm
contact affiliation	Monash University
contact email	ralf.schittenhelm@monash.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD028332

PRIDE project URI

• PRIDE
  1. PXD028332
     1. Label: PRIDE project
     2. Name: Antibiotic-Chemoattractants Enhance Neutrophil Clearance of Staphylococcus aureus