PXD028322

PXD028322 is an original dataset announced via ProteomeXchange.

Title	Transcriptome and proteome analysis in LUHMES cells overexpressing alpha-synculein
Description	LUHMES cells share many characteritics with human dopamingeric neurons in the substantia nigra, the cells whose demise is responsible for the motor symptoms in Parkinson’s disease (PD). LUHMES cells can therefore be used bona fide as a model to study pathophysiological processes involved in PD. Previously, we showed that LUHMES cell degenerate after six days upon overexpression of wild type alpha-synuclein. In the present study we performed a transcriptome and proteome expression analysis in alpha-synuclein-overexpressing cells and GFP-expressing control cells in order to identify genes and proteins that are differentially regulated upon overexpression of alpha-synuclein. The analysis was performed four days after the initiation of alpha-synuclein or GFP overexpression, before the cells died in order to identify processes that preceded cell death.
HostingRepository	PRIDE
AnnounceDate	2022-05-19
AnnouncementXML	Submission_2022-05-19_08:54:05.949.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD028322
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Markus Stepath
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-09 08:25:23	ID requested
⏵ 1	2022-05-19 08:54:06	announced

H, ö, llerhage M, Stepath M, Kohl M, Pfeiffer K, Chua OWH, Duan L, Hopfner F, Eisenacher M, Marcus K, H, ö, glinger GU, Transcriptome and Proteome Analysis in LUHMES Cells Overexpressing Alpha-Synuclein. Front Neurol, 13():787059(2022) [pubmed]

submitter keyword: alpha-synculein, SNCA, proteome, Transcriptome, LUHMES cells, Neurodegenerative Diseases, Parkinson’s disease (PD)

PD Dr. Martin Eisenacher
contact affiliation	Medical Bioinformatics, Medizinisches Proteom-Center, Medical Faculty, Ruhr University Bochum, Bochum, Germany
contact email	martin.eisenacher@ruhr-uni-bochum.de
lab head
Markus Stepath
contact affiliation	Ruhr University Bochum - Medizinisches Proteom-Center
contact email	markus.stepath@rub.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/05/PXD028322

PRIDE project URI

• PRIDE
  1. PXD028322
     1. Label: PRIDE project
     2. Name: Transcriptome and proteome analysis in LUHMES cells overexpressing alpha-synculein