PXD028319

PXD028319 is an original dataset announced via ProteomeXchange.

Title	Arabidopsis SLOMO-Decoy line AP-MS
Description	SLOMO is an F-box protein, but little is known about its function and interactors as an F-box protein. we used a SLOMO-decoy approach here. The SLOMO-decoy protein, which lacks the F-box domain, is still able to bind to substrates but lacks the ability to recruit E2 conjugating enzymes to mediate substrate ubiquitination and degradation. We generated a stable Arabidopsis line expressing 35S::GFP:SLOMO-Decoy, and performed immunoprecipitation mass spectrometry (IP-MS) analyses on GFP:SLOMO-decoy.
HostingRepository	PRIDE
AnnounceDate	2025-05-26
AnnouncementXML	Submission_2025-05-25_16:07:52.525.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shanshuo Zhu
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-09 08:25:08	ID requested
⏵ 1	2025-05-25 16:07:53	announced

10.1111/nph.19383;

Zhu S, Pan L, Vu LD, Xu X, Orosa-Puente B, Zhu T, Neyt P, van de Cotte B, Jacobs TB, Gendron JM, Spoel SH, Gevaert K, De Smet I, Phosphoproteome analyses pinpoint the F-box protein SLOW MOTION as a regulator of warm temperature-mediated hypocotyl growth in Arabidopsis. New Phytol, 241(2):687-702(2024) [pubmed]

submitter keyword: AP-MS, SLOMO-Decoy,Arabidopsis,GFP trap

Ive De Smet
contact affiliation	Plant system biolog of Ugent-VIB
contact email	Ive.DeSmet@psb.ugent.be
lab head
Shanshuo Zhu
contact affiliation	VIB-Ugent center for Plant systems biology
contact email	shzhu@psb.vib-ugent.be
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD028319

PRIDE project URI

• PRIDE
  1. PXD028319
     1. Label: PRIDE project
     2. Name: Arabidopsis SLOMO-Decoy line AP-MS