PXD028315

PXD028315 is an original dataset announced via ProteomeXchange.

Title	De novo formation of apical-basal polarity by Rap1 regulates epiblast patterning
Description	The epiblast is the first cell type that forms apical-basal polarity de novo as the mouse embryo implants into the maternal uterus, while the extraembryonic neighbours of the epiblast - trophectoderm and primitive endoderm - retain their pre-established polarity beyond implantation [1]; however, it is still unclear how the epiblast establishes apical-basal polarity de novo. Here, we focused on Rap1 signaling pathway, which is activated during the transition of the epiblast from the naïve to primed state of pluripotency during implantation [2]. Through the preestablished in vitro three-dimensional culture system [3], genetic knockouts and proximity-biotinylation analyses, we found that Rap1 integrates multiple signals that contribute to de novo formation of apical-basal polarity. Importantly, formation of apical-basal polarity in the epiblast is essential for its correct patterning and proper communication with the extraembryonic lineages. Altogether, these results not only dissect molecular details of de novo apical-basal polarity formation, but also have broader implications for epithelial polarity and development.
HostingRepository	PRIDE
AnnounceDate	2022-06-14
AnnouncementXML	Submission_2022-06-14_08:44:25.648.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hannes Drexler
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-09 08:21:37	ID requested
⏵ 1	2022-06-14 08:44:26	announced

Dataset with its publication pending

submitter keyword: Rap1, development, epiblast, apical - basal polarity, proteomics, LC-MSMS, proximity labeling, Biotin

Hannes C. A. Drexler
contact affiliation	Max Planck Institute for Moleclar Biomedicine Bioanalyticyl Mass Spectrometry Unit Röntgenstr. 20 48149 Münster Germany
contact email	hannes.drexler@mpi-muenster.mpg.de
lab head
Hannes Drexler
contact affiliation	Bioanalytical Mass Spectrometry
contact email	hannes.drexler@mpi-muenster.mpg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD028315
     1. Label: PRIDE project
     2. Name: De novo formation of apical-basal polarity by Rap1 regulates epiblast patterning