PXD028308

PXD028308 is an original dataset announced via ProteomeXchange.

Title	AP-MS of replisomes from S. cerevisiae in S phase and after unscheduled G1 replication
Description	Interfering with once-per-cell-cycle regulation of DNA replication initiation generates genome instability through over-replication and has been linked to early stages of cancer development. Here, we engineered different systems in budding yeast to induce over-replication in G1 and investigated its characteristics and consequences within the same cell cycle. To study replisome composition, we immunopurified replisomes via a GFP-tag on the Psf2 subunit of the GINS complex from S phase in the presence and absence of hydroxyurea and after unscheduled replication initiation in G1.
HostingRepository	PRIDE
AnnounceDate	2022-11-09
AnnouncementXML	Submission_2022-11-08_23:53:31.807.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	MichaelWierer
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-09 08:20:53	ID requested
⏵ 1	2022-11-08 23:53:32	announced

Dataset with its publication pending

submitter keyword: baker’s yeast, cell division cycle, replication initiation, re-replication, replication stress, replisome

MatthiasMann
contact affiliation	Department of Proteomics and Signal Transduction, Max-Planck-Institute of Biochemistry, Munich, Germany
contact email	mmann@biochem.mpg.de
lab head
MichaelWierer
contact affiliation	Department of Proteomics and Signal Transduction, Max-Planck Institute of Biochemistry, Martinsried, Germany
contact email	wierer@biochem.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/11/PXD028308

PRIDE project URI

• PRIDE
  1. PXD028308
     1. Label: PRIDE project
     2. Name: AP-MS of replisomes from S. cerevisiae in S phase and after unscheduled G1 replication